The responses of indigenous oil-degrading bacteria to oil exposure in Phragmites australis-dominated marsh soil: a mesocosm study
文献类型：期刊论文
| 作者 | Wang, Jiaoyue1,2,3; Judy, Chad Robert4; Hou, Aixin2,4 |
| 刊名 | HYDROBIOLOGIA
 |
| 出版日期 | 2019 |
| 卷号 | 827期号:1页码:65-74 |
| 关键词 | Deepwater Horizon oil spill Phragmites australis Alkane monooxygenase (alkB) PAH-ring hydroxylating dioxygenase alpha subunit (PAH-RHD) Real-time PCR Gene expression |
| ISSN号 | 0018-8158 |
| DOI | 10.1007/s10750-017-3461-3 |
| 英文摘要 | This study examined impacts of Macondo MC252 oil from the Deepwater Horizon spill on oil-degrading bacteria in fresh and oligohaline marshes dominated by the common reed Phragmites australis (Cav.) Trin. ex Steud. and correlated microbial changes to soil variables. We hypothesized that indigenous oil-degrading bacteria and soil characteristics in the marshes effectively respond to Macondo oil. We tested this hypothesis with a greenhouse mesocosm study. Weathered and emulsified Macondo oil was applied to P. australis sods at different rates (0, 4, 8, 12, and 16Lm(-2)). Populations of oil degrading bacteria containing alkane monooxygenase and PAH-ring hydroxylating dioxygenase alpha subunit (PAH-RHD) genes, the expression of these genes, soil respiration rate, residual total petroleum hydrocarbon, redox potential (Eh), as well as dissolved organic carbon (DOC) and its aromaticity index of SUVA(254) were measured in soils 2months after oil addition. Oil exposure generally increased oil-degrading bacterial populations, in particular Gram-positive (GP) PAH-degraders, soil respiration rate, DOC concentration, and aromaticity of DOC, but decreased Eh values. GP PAH-RHD genes accounted for approximately 98% of total detected genes, and expression of these genes increased by a factor of 3 to 5 at various oil dosages. Both abundance and expression of GP PAH-RHD genes significantly correlated to SUVA(254) (P<0.05). The study revealed that indigenous oil-degrading bacteria effectively responded to weathered Macondo oil in the P. australis marsh soil wherein GP bacteria with PAH-RHD genes played a major role in biodegradation of PAH-enriched petroleum hydrocarbons. |
| 资助项目 | Gulf of Mexico Research Initiative (GoMRI) ; BP/GoMRI through the Northern Gulf Institute ; National Natural Science Foundation of China[41671105] ; National Natural Science Foundation of China[41603068] |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000451426200006 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/123767]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Hou, Aixin |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Changchun 130102, Jilin, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 4.Louisiana State Univ, Dept Environm Sci, Coll Coast & Environm, Baton Rouge, LA 70803 USA |
| 推荐引用方式 GB/T 7714 | Wang, Jiaoyue,Judy, Chad Robert,Hou, Aixin. The responses of indigenous oil-degrading bacteria to oil exposure in Phragmites australis-dominated marsh soil: a mesocosm study[J]. HYDROBIOLOGIA,2019,827(1):65-74. |
| APA | Wang, Jiaoyue,Judy, Chad Robert,&Hou, Aixin.(2019).The responses of indigenous oil-degrading bacteria to oil exposure in Phragmites australis-dominated marsh soil: a mesocosm study.HYDROBIOLOGIA,827(1),65-74. |
| MLA | Wang, Jiaoyue,et al."The responses of indigenous oil-degrading bacteria to oil exposure in Phragmites australis-dominated marsh soil: a mesocosm study".HYDROBIOLOGIA 827.1(2019):65-74. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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