Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746
文献类型：期刊论文
| 作者 | Pan, Huaqi1,2; Tian, Xinpeng2; Shao, Mingwei2; Xie, Yunchang2; Huang, Hongbo2; Hu, Jiangchun1; Ju, Jianhua2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 103期号:10页码:4153-4165 |
| 关键词 | Bacillus Genome Secondary metabolite Siderophore Non-ribosomal peptide |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-019-09759-2 |
| 英文摘要 | Bacillus spp. are important producers of bioactive natural products with potential applications in medicine and agriculture. Bacillus sp. SCSIO 05476 from a deep-sea sediment exhibits broad-spectrum antimicrobial activities and strong cytotoxic activity. Here, an integrative approach combining genome mining and metabolic profiling has been applied to decipher the chemical origins of this strain's varied and significant biological activities. First, genome mining revealed 19 candidate gene clusters encoding the biosynthesis of diverse secondary metabolites. Then, a series of bacillibactins, fengycins, bacillomycins, surfactins, bacillaenes, macrolactins, and related species were found by LC-DAD-MS. Finally, three new linear bacillibactins, linbacillibactins A-C (1-3), along with 11 known secondary metabolites, bacillibactin (4), normal-C-13 Val(7) surfactin (5), anteiso-C-13 Leu(7) surfactin (6), iso-C-14 Leu(7) surfactin (7), normal-C-14 Leu(7) surfactin (8), anteiso-C-14 Leu(7) surfactin (9), macrolactin D (10), normal-C-14 bacillomycin D (11), iso-C-16 bacillomycin D (12), normal-C-17 bacillomycin D (13), and iso-C-17 bacillomycin D (14), were obtained and elucidated by bioactivity and structure-guided isolation from the fermentation of strain SCSIO 05746. Among them, new compounds 1-3 show significant siderophore activities comparable to that of bacillibactin (4), compounds 13 and 14 exhibit strong cytotoxic activity. At the same time, the strain classification status was confirmed by genomic analyses, and the complete genome sequence of Bacillus siamensis was presented firstly. This study provides a foundation for understanding the mechanisms driving SCSIO 05746's multiple bioactivities and demonstrates a successful way of discovering bioactive metabolites using a combination of genome mining and metabolic profiling methods. |
| 资助项目 | National Natural Science Foundation of China[41576136] ; National Natural Science Foundation of China[81425022] ; National Natural Science Foundation of China[31872036] ; National Natural Science Foundation of China[U1501223] ; Key Laboratory of Tropical Marine Bio-resources and Ecology Program of CAS[LMB151005] ; Science and Technology Innovation Program for the Youth Talents of Shenyang[RC170266] ; Youth Innovation Promotion Association CAS[2018229] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000466111500018 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/124057]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Ju, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Huaqi,Tian, Xinpeng,Shao, Mingwei,et al. Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(10):4153-4165. |
| APA | Pan, Huaqi.,Tian, Xinpeng.,Shao, Mingwei.,Xie, Yunchang.,Huang, Hongbo.,...&Ju, Jianhua.(2019).Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(10),4153-4165. |
| MLA | Pan, Huaqi,et al."Genome mining and metabolic profiling illuminate the chemistry driving diverse biological activities of Bacillus siamensis SCSIO 05746".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.10(2019):4153-4165. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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