Evaluation of 10 plant barcodes in Bryophyta (Mosses)
文献类型：期刊论文
| 作者 | Liu, Y; Yan, HF; Cao, T; Ge, XJ; Cao, T, Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China |
| 刊名 | JOURNAL OF SYSTEMATICS AND EVOLUTION
 |
| 出版日期 | 2010 |
| 卷号 | 48期号:1页码:36-46 |
| 关键词 | Bryophyta Dna Barcoding Rbcl Rpoc1 Rps4 Trnh-psba Trnl-trnf |
| ISSN号 | 0529-1526 |
| 英文摘要 | DNA barcoding is a molecular tool that uses a standardized DNA region to identify species. Our preliminary study reported here is the first attempt to specifically focus on universality and attributes of candidate barcodes across a wide systematic range of mosses. We tested eight previously proposed plant barcoding regions (atpf-atpH, ITS2, matK, psbK-psbI, rbcL, rpoB, rpoC1, and trnH-psbA) and two popular phylogenetic markers (rps4 and trnL-trnF of cpDNA) in 49 moss species and 9 liverwort species, representing half of the orders in moss lineages. The ITS2, rbcL, rpoC1, rps4, trnH-psbA and trnL-trnF regions showed good universality, and therefore the efficacy of these loci as DNA barcodes was further evaluated in 36 mosses and 2 liverworts, each of which included two to three individuals per taxa. The five loci, viz. rbcL, rpoC1, rps4, trnH-psbA and trnL-trnF, were easy to amplify and sequence and showed significant inter-specific genetic variability, making them potentially useful DNA barcodes for mosses. The best performing single loci were the rbcL and rpoC1 coding regions. Several loci showed equivalent performance and combinations of them did not greatly increase their discrimination capacity. In addition, phylogenies generated from each of the separate regions and multi-locus combinations by using best-fit and Kimura 2-parameter models were compared, but no significant difference was found. |
| 学科主题 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000274470600002 |
| 资助机构 | Chinese Academy of Sciences [2009-LSF-GBOWS-01]; Natural Science Foundation of China [30770141]; Shanghai Leading Academic Discipline Project [S30406] |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/53923]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Cao, T, Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Yan, HF,Cao, T,et al. Evaluation of 10 plant barcodes in Bryophyta (Mosses)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2010,48(1):36-46. |
| APA | Liu, Y,Yan, HF,Cao, T,Ge, XJ,&Cao, T, Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China.(2010).Evaluation of 10 plant barcodes in Bryophyta (Mosses).JOURNAL OF SYSTEMATICS AND EVOLUTION,48(1),36-46. |
| MLA | Liu, Y,et al."Evaluation of 10 plant barcodes in Bryophyta (Mosses)".JOURNAL OF SYSTEMATICS AND EVOLUTION 48.1(2010):36-46. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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