Biological characterization of the zinc site coordinating histidine residues of staphylococcal enterotoxin C2
文献类型：期刊论文
| 作者 | Wang, XG; Zhang, HW; Xu, MK; Cai, YM; Liu, CX; Su, ZC; Zhang, CG; Xu, MK, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2009 |
| 卷号 | 155期号:22页码:680-686 |
| ISSN号 | 1350-0872 |
| 英文摘要 | The bacterial toxin staphylococcal enterotoxin C2 (SEC2) can cause staphylococcal toxic shock syndrome and food poisoning. Although the previously determined crystal structure of SEC2 revealed that some histidine residues (His47, Hisl 18 and Hisl 22) contribute to the binding of zinc ions, little is known about their biological roles in SEC2. This prompted us to investigate the role of the zinc site coordinating histidine residues in the biological activities of SEC2. The mutants with substitutions at positions 118 and 122 all retained T-cell stimulatory activity, whereas the histidine mutants at position 47 were defective in the ability to stimulate T-cell proliferation. Further toxicity assays in vivo indicated that mutants SEC2-H118A and SEC2-H122A were defective in emetic and febrile activities. However, mutant SEC2-H47A could cause significant emetic and febrile responses in comparison with the other two histidine mutants. These findings suggested that the zinc-coordinating histidine residues play significant roles in superantigen and toxic activities of SEC2 and further implied that superantigen and febrile activities could be separable in staphylococcal enterotoxins. The results also show that it should be possible to design new SEC2 immunotherapeutic agents that have superantigen activity and low toxicity. |
| 学科主题 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264515100004 |
| 资助机构 | Sciential Innovation Project of the Institute of Applied Ecology of the CAS [06LYQYC001]; Shenyang Xiehe pio-pharmaceutical Co. Ltd |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54191]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Xu, MK, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, XG,Zhang, HW,Xu, MK,et al. Biological characterization of the zinc site coordinating histidine residues of staphylococcal enterotoxin C2[J]. MICROBIOLOGY-SGM,2009,155(22):680-686. |
| APA | Wang, XG.,Zhang, HW.,Xu, MK.,Cai, YM.,Liu, CX.,...&Xu, MK, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2009).Biological characterization of the zinc site coordinating histidine residues of staphylococcal enterotoxin C2.MICROBIOLOGY-SGM,155(22),680-686. |
| MLA | Wang, XG,et al."Biological characterization of the zinc site coordinating histidine residues of staphylococcal enterotoxin C2".MICROBIOLOGY-SGM 155.22(2009):680-686. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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