Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils
文献类型：期刊论文
| 作者 | Li, XY; Zhang, HW; Su, ZC; Zhang, CG; Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Microbiolog Resource & Ecol Grp, Shenyang 110016, Peoples R China; Wu, M |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:9页码:1126-1131 |
| 关键词 | Ammonia-oxidizing Bacteria (Aob) Pcr-dgge Acetochlor |
| ISSN号 | 1001-0742 |
| 英文摘要 | Acetochlor is an increasingly used herbicide on corn in North China. Currently, the effect of acetochlor on soil ammonia-oxidizing bacteria (AOB) communities is not well documented. Here, we studied the diversity and community composition of AOB in soil amended with three concentrations of acetochlor (50, 150, 250 mg/kg) and the control (0 mg acetochlor/kg soil) in a microcosm experiment by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and the phylogenetic analysis of excised DGGE bands. DGGE profiles showed that acetochlor had a stimulating effect on AOB at the early stage after acetochlor amended, and the order of intensity and duration is medium-acetochlor amended samples (AM) > low-acetochlor amended samples (AL) > high-acetochlor amended samples (AH). At the end of 60 d microcosm, acetochlor had a negative effect on the diversity of AOB. Cluster analysis of DGGE profiles showed that acetochlor had a greater effect on the community structure of AOB on day 60 than on day 1. The phylogenetic analysis revealed that all the sequences of excised DGGE bands were closely related to members of the genus Nitrosospira and formed two separate subclusters designated as subcluster 1 and subcluster 2 affiliated respectively with Clusters 3 and 4 in Nitrosospira as defined by Stephen. Some dominant AOB had a change from subcluster 2 to subcluster I with the incubation. The results showed that acetochlor had an effect on the AOB on a long-term basis and the chronic effect of acetochlor should be paid more attention in future. |
| 学科主题 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000259244600016 |
| 资助机构 | Natural Science Foundation for Young Scientists of China [40701088] |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54387]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Microbiolog Resource & Ecol Grp, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, XY,Zhang, HW,Su, ZC,et al. Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(9):1126-1131. |
| APA | Li, XY,Zhang, HW,Su, ZC,Zhang, CG,Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Microbiolog Resource & Ecol Grp, Shenyang 110016, Peoples R China,&Wu, M.(2008).Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(9),1126-1131. |
| MLA | Li, XY,et al."Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.9(2008):1126-1131. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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