A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers
文献类型：期刊论文
| 作者 | Zhang, XD; He, HB; Amelung, W; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:11页码:2785-2796 |
| 关键词 | Amino Acid Enantiomer C-13 N-15 Incubation Gc/ms Atom Percentage Excess (Ape) |
| ISSN号 | 0038-0717 |
| 英文摘要 | Identifying the transformation process of amino acid enantionters was essential to probe into the fate, turnover and aging of soil nitrogen due to their important roles in the biogeochemical cycling. If this can be achieved by differentiating between the newly biosynthesized and the inherent compounds in soil, then the isotope tracer method can be considered most valid. We thereby developed a gas chromatography/mass spectrometry (GC/MS) method to trace the N-15 or C-13 isotope incorporation into soil amino acid enantiomers after being incubated with (NH4+)-N-15 or U-C-13-glucose substrates. The most significant fragments (F) as well as the related minor ions were monitored by the full scan mode and the isotope enrichment in amino acids was estimated by calculating the atom percentage excess (APE). (NH4+)-N-15 incorporation was evaluated according to the relative abundance increase of m/z F + I to F for neutral and acidic amino acids and F + 2 to F (mass 439) for lysine. The assessment of C-13 enrichment in soil amino acids was more complicated than that of N-15 due to multi-carbon atoms in amino acid molecules. The abundance ratio increment of m/z F + n to F (n is the original skeleton carbon number in each fragment) indicated the direct conversion from the added glucose to amino acids, but the total isotope incorporation from the added C-13 can only be calculated according to all target isotope fragments, i.e. the abundance ratio increment summation from m/z (Fa + 1) through m/z (Fa + T) represented the total incorporation of the added C-13 (Fa is the fragment containing all original skeleton carbons and T is the carbon number in the amino acid molecule). This method has a great advantage especially for the evaluation of high-abundance isotope enrichment in organic compounds compared with GC/C/IRMS. And in principle, this technique is also valid for amino acids besides enantiomers if stereoisomers are not concerned. Our assessment approach could shine a light on investigating the biochemical mechanism of microbial transformation of N and C in soils of terrestrial ecosystem. (c) 2007 Elsevier Ltd. All rights reserved. |
| 学科主题 | Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000249538500010 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54617]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XD,He, HB,Amelung, W,et al. A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers[J]. SOIL BIOLOGY & BIOCHEMISTRY,2007,39(11):2785-2796. |
| APA | Zhang, XD,He, HB,Amelung, W,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2007).A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers.SOIL BIOLOGY & BIOCHEMISTRY,39(11),2785-2796. |
| MLA | Zhang, XD,et al."A GC/MS method for the assessment of N-15 and C-13 incorporation into soil amino acid enantiomers".SOIL BIOLOGY & BIOCHEMISTRY 39.11(2007):2785-2796. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。