Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates
文献类型：期刊论文
| 作者 | Zhang, W; He, HB; Zhang, XD; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2007 |
| 卷号 | 39期号:10页码:2665-2669 |
| 关键词 | Aldononitrile Acetate Gas Chromatography Soil Neutral Sugars Tfa Hydrolysis Determination |
| ISSN号 | 0038-0717 |
| 英文摘要 | We have quantified ribose, rhamnose, arabinose, xylose, fucose, mannose, glucose, and galactose in soil by gas chromatography (GC) simultaneously after converting to aldononitrile acetate derivatives. A recommended single-hydrolytic step by 4M trifluoroacetic acid (TFA) at 105 degrees C for 4 h was more effective for releasing soil neutral sugars from non-cellulosic carbohydrates and better suited to our purification procedure compared with the sulphuric acid hydrolysis. Linearity of the GC detection for each neutral sugar was in the range of 10-640 mu gml(-1) and the recovery of neutral sugars from the spiked soil samples ranged from 76% to 109.7%. The coefficients of variation of the neutral sugars in four soils were lower than 2.0% for the instrument and 4.6-7.6% for the whole determination procedures. Compared with the trimethylsilyl JMS) derivatization, the recovery of our newly modified method was more satisfactory and the reproducibility of ribose was improved significantly. Moreover, the aldononitrile acetate derivative was more stable than TMS derivative. Therefore, it is a prornising approach suitable for a routine use in the quantitative analysis of soil neutral sugars, since it is fast, sensitive, and reproducible. (c) 2007 Elsevier Ltd. All rights reserved. |
| 学科主题 | Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000248822500025 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54647]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, W,He, HB,Zhang, XD,et al. Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates[J]. SOIL BIOLOGY & BIOCHEMISTRY,2007,39(10):2665-2669. |
| APA | Zhang, W,He, HB,Zhang, XD,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2007).Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates.SOIL BIOLOGY & BIOCHEMISTRY,39(10),2665-2669. |
| MLA | Zhang, W,et al."Determination of neutral sugars in soil by capillary gas chromatography after derivatization to aldononitrile acetates".SOIL BIOLOGY & BIOCHEMISTRY 39.10(2007):2665-2669. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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