Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil
文献类型：期刊论文
| 作者 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China; Zhang, CG; Li, XY; Zhang, Q; Zhang, HW; Su, ZC |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2007 |
| 卷号 | 19期号:7页码:864-873 |
| 关键词 | Agrochemicals Acetochlor Methamidophos Toxic Effects Nifh Gene Pcr-dgge |
| ISSN号 | 1001-0742 |
| 英文摘要 | Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity (both in gene richness and diversity index values) and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor (50 mg/kg) recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos (150 mg/kg) caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos (250 mg/kg) produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes (bands) responded differently to the impact of agrochemicals: four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera. |
| 学科主题 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000248095400016 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54669]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China,Zhang, CG,Li, XY,et al. Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2007,19(7):864-873. |
| APA | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China,Zhang, CG,Li, XY,Zhang, Q,Zhang, HW,&Su, ZC.(2007).Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,19(7),864-873. |
| MLA | Zhang, HW, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China,et al."Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 19.7(2007):864-873. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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