Optimization of laccase production from Trametes versicolor by solid fermentation
文献类型：期刊论文
| 作者 | Jing, DB; Li, PJ; Stagnitti, F; Xiong, XZ; Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China |
| 刊名 | CANADIAN JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 53期号:2页码:245-251 |
| 关键词 | Trametes Versicolor Laccase Formulation Experiment Solid Fermentation Uniform Design |
| ISSN号 | 0008-4166 |
| 英文摘要 | The regulation of culture conditions, especially the optimization of substrate constituents, is crucial for laccase production by solid fermentation. To develop an inexpensive optimized substrate formulation to produce high-activity laccase, a uniform design formulation experiment was devised. The solid fermentation of Trametes versicolor was performed with natural aeration, natural substrate pH (about 6.5), environmental humidity of 60% and two different temperature stages (at 37 degrees C for 3 days, and then at 30 degrees C for the next 17 days). From the experiment, a regression equation for laccase activity, in the form of a second-degree polynomial model, was constructed using multivariate regression analysis and solved with unconstrained optimization programming. The optimized substrate formulation for laccase production was then calculated. Tween 80 was found to have a negative effect on laccase production in solid fermentation; the optimized solid substrate formulation was 10.8% glucose, 27.7% wheat bran, 9.0% (NH4)(2)SO4, and 52.5% water. In a scaled-up verification of solid fermentation at a 10 kg scale, laccase activity from T. versicolor in the optimized substrate formulation reached 110.9 IU/g of dry mass. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246963200011 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54701]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, DB,Li, PJ,Stagnitti, F,et al. Optimization of laccase production from Trametes versicolor by solid fermentation[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2007,53(2):245-251. |
| APA | Jing, DB,Li, PJ,Stagnitti, F,Xiong, XZ,&Jing, DB, Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China.(2007).Optimization of laccase production from Trametes versicolor by solid fermentation.CANADIAN JOURNAL OF MICROBIOLOGY,53(2),245-251. |
| MLA | Jing, DB,et al."Optimization of laccase production from Trametes versicolor by solid fermentation".CANADIAN JOURNAL OF MICROBIOLOGY 53.2(2007):245-251. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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