Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108
文献类型：期刊论文
| 作者 | Shi, SL; Pan, MH; Lu, C; Lu, C, Southwest Univ, Key Sericultural Lab, Agr Minist, Coll Sericulture & Biotechnol, Chongqing 400716, Peoples R China |
| 刊名 | VIRUS GENES
 |
| 出版日期 | 2007 |
| 卷号 | 35期号:1页码:97-101 |
| 关键词 | Antheraea Pernyi Baculovirus Gene Expression Nucleopolyhedrovirus P11 Gene Orf108 |
| ISSN号 | 0920-8569 |
| 英文摘要 | Antheraea pernyi nucleopolyhedrovirus (ApNPV) p11 gene is 309 bp long, potentially encoding 102 amino acids with a predicted molecular weight of 11.2 kDa. ApNPV p11 gene was cloned into the prokaryotic expression vector pQE-30 and P11 was expressed in E. coli M15. Polyclonal antiserum was made against 6xHis tagged P11 protein expressed in E. coli M15. P11 gene transcription was detected as early as 36 h post-infection (p.i.) in tussah pupa and remain at high level up to 96 h p.i. Structural localization revealed that P11 protein was present in polyhedral inclusion bodies (PIB) dilute alkaline saline (DAS) pellet (P) fractions and occlusion-derived virus (ODV), but not in PIB DAS supernatant (S) fractions and budded virus (BV). These results indicated that P11 was associated with ApNPV structure. |
| 学科主题 | Genetics & Heredity ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246521900012 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54707]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Lu, C, Southwest Univ, Key Sericultural Lab, Agr Minist, Coll Sericulture & Biotechnol, Chongqing 400716, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, SL,Pan, MH,Lu, C,et al. Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108[J]. VIRUS GENES,2007,35(1):97-101. |
| APA | Shi, SL,Pan, MH,Lu, C,&Lu, C, Southwest Univ, Key Sericultural Lab, Agr Minist, Coll Sericulture & Biotechnol, Chongqing 400716, Peoples R China.(2007).Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108.VIRUS GENES,35(1),97-101. |
| MLA | Shi, SL,et al."Characterization of Antheraea pernyi nucleopolyhedrovirus p11 gene, a homologue of Autographa californica nucleopolyhedrovirus orf108".VIRUS GENES 35.1(2007):97-101. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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