Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure
文献类型：期刊论文
| 作者 | Liu, M; Zhang, W; Song, YF; Gong, P; Sun, TH; Zhou, QX; Song, YF, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 刊名 | JOURNAL OF ENVIRONMENTAL MONITORING
 |
| 出版日期 | 2006 |
| 卷号 | 8期号:9页码:963-967 |
| ISSN号 | 1464-0325 |
| 英文摘要 | We developed a new microsome purification method and used the Omura and Sato method to measure the total content of cytochrome (Cyt) P450 in earthworm (Eisenia fetida) microsomes. In method development, two different pretreatments, i. e. solubilization or manual separation were used to purify worm microsomes. Solubilization was more effective than manual separation and difference spectra showed a peak at 450 +/- 1 nm in microsomes received solubilization pretreatment. We conducted a 48 h contact test by exposing worms on pyrene (Py) and benzo[ a] pyrene (BaP) spiked filter paper. A dose-response relationship was established between total P450 content and the concentration of Py or BaP ranging from 10(-6) mg mL(-1) to 10(-2) mg mL(-1). Results show that total Cyt P450 content in earthworms is a promising biomarker for diagnosing PAHs exposure at sublethal dose ranges. |
| 学科主题 | Chemistry, Analytical ; Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000240236200013 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54885]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Song, YF, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, M,Zhang, W,Song, YF,et al. Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure[J]. JOURNAL OF ENVIRONMENTAL MONITORING,2006,8(9):963-967. |
| APA | Liu, M.,Zhang, W.,Song, YF.,Gong, P.,Sun, TH.,...&Song, YF, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2006).Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure.JOURNAL OF ENVIRONMENTAL MONITORING,8(9),963-967. |
| MLA | Liu, M,et al."Earthworm cytochrome P450 determination and application as a biomarker for diagnosing PAH exposure".JOURNAL OF ENVIRONMENTAL MONITORING 8.9(2006):963-967. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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