Fusion immunotoxin anti-HER-2-scFv-SEC2 expressed in E-coli with an improved expression vector pASK75-EX: its construction and functioning
文献类型：期刊论文
| 作者 | Zhang, XE; Xu, MK; Zhang, CG; Liu, L; Zhang, HW; Zhou, YF; Zhang, CG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2006 |
| 卷号 | 33期号:8页码:781-788 |
| 关键词 | Staphylococcal Enterotoxin C2 (seC2) Her-2 Fusion Immunotoxin Tumor- Targeting Pask75 |
| ISSN号 | 1000-3282 |
| 英文摘要 | A tumor-targeting recombinant fusion immunotoxin B-L-SEC2 was constructed by fusing staphylococcal enterotoxin C2 (SEC2) and an anti-HER-2 single-chain Fv B1 through a peptide linker, and expressed in E.coli strain BL21 (DE3) with an improved expression vector pASK75-EX as inclusion body. The denatured inclusion body was purified with Ni-NTA chelate agarose, and then re-natured by dialysis. FACS and MTT assays indicated that the re-natured fusion immunotoxin B-L-SEC2 could target the HER-2 over-expressing breast tumor cell SK-Br-3 in vitro, and inhibit the growth of SK-Br-3. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000240004100011 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54889]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, CG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XE,Xu, MK,Zhang, CG,et al. Fusion immunotoxin anti-HER-2-scFv-SEC2 expressed in E-coli with an improved expression vector pASK75-EX: its construction and functioning[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(8):781-788. |
| APA | Zhang, XE.,Xu, MK.,Zhang, CG.,Liu, L.,Zhang, HW.,...&Zhang, CG, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2006).Fusion immunotoxin anti-HER-2-scFv-SEC2 expressed in E-coli with an improved expression vector pASK75-EX: its construction and functioning.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(8),781-788. |
| MLA | Zhang, XE,et al."Fusion immunotoxin anti-HER-2-scFv-SEC2 expressed in E-coli with an improved expression vector pASK75-EX: its construction and functioning".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.8(2006):781-788. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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