Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment
文献类型：期刊论文
| 作者 | Jing, DB; Li, PJ; Stagnitti, F; Xiong, XZ; Li, L; Stagnitti, F, Deakin Univ, Sch Ecol & Environm, Warrnambool Campus,POB 432, Geelong, Vic 3280, Australia |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2006 |
| 卷号 | 64期号:2页码:244-250 |
| 关键词 | Aspergillus Niger Pectinase Solid Fermentation Substrate Prescription Experiment Multiple Regression |
| ISSN号 | 0147-6513 |
| 英文摘要 | The Ordos Plateau in China is covered with up to 300,000 ha of peashrub (Caragana) which is the dominant natural vegetation and ideal for fodder production. To exploit peashrub fodder, it is crucially important to optimize the culture conditions, especially culture substrate to produce pectinase complex. In this study, a new prescription process was developed. The process, based on a uniform experimental design, first optimizes the solid substrate and second, after incubation, applies two different temperature treatments (30 degrees C for the first 30 h and 23 degrees C for the second 42 h) in the fermentation process. A multivariate regression analysis is applied to a number of independent variables (water, wheat bran, rice dextrose, ammonium sulfate, and Tween 80) to develop a predictive model of pectinase activity. A second-degree polynomial model is developed which accounts for an excellent proportion of the explained variation (R-2 = 97.7%). Using unconstrained mathematical programming, an optimized substrate prescription for pectinase production is subsequently developed. The mathematical analysis revealed that the optimal formula for pectinase production from Aspergillus niger by solid fermentation under the conditions of natural aeration, natural substrate pH (about 6.5), and environmental humidity of 60% is rice dextrose 8%, wheat bran 24%, ammonium sulfate ((NH4)(2)SO4) 6%, and water 61%. Tween 80 was found to have a negative effect on the production of pectinase in solid substrate. With this substrate prescription, pectinase produced by solid fermentation of A. niger reached 36.3IU/(gDM). Goats fed on the pectinase complex obtain an incremental increase of 0.47 kg day(-1) during the initial 25 days of feeding, which is a very promising new feeding prospect for the local peashrub. It is concluded that the new formula may be very useful for the sustainable development of and and semiarid pastures such as those of the Ordos Plateau. (c) 2005 Elsevier Inc. All rights reserved. |
| 学科主题 | Environmental Sciences ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000238404300019 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/54951]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Stagnitti, F, Deakin Univ, Sch Ecol & Environm, Warrnambool Campus,POB 432, Geelong, Vic 3280, Australia |
| 推荐引用方式 GB/T 7714 | Jing, DB,Li, PJ,Stagnitti, F,et al. Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2006,64(2):244-250. |
| APA | Jing, DB,Li, PJ,Stagnitti, F,Xiong, XZ,Li, L,&Stagnitti, F, Deakin Univ, Sch Ecol & Environm, Warrnambool Campus,POB 432, Geelong, Vic 3280, Australia.(2006).Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,64(2),244-250. |
| MLA | Jing, DB,et al."Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 64.2(2006):244-250. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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