DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis
文献类型：期刊论文
| 作者 | Liu, W; Li, PJ; Qi, XM; Zhou, Q; Zheng, L; Sun, TH; Yang, YS; Liu, W, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2005 |
| 卷号 | 61期号:2页码:158-167 |
| 关键词 | Cadmium (Cd) Root Growth Total Soluble Protein Content Dna Polymorphism Change Genomic Template Stability Biomarker |
| ISSN号 | 0045-6535 |
| 英文摘要 | In recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, barley (Hordeum vulgare L) seedling was used as bioindicator of cadmium (Cd) pollution in the range of 30-120 mg l(-1). Inhibition of root growth and reduction of total soluble protein content in root tips of barley seedlings were observed with the increase of Cd concentrations. The changes occurring in random amplified polymorphic DNA (RAPD) profiles of root tips following Cd treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the normal seedlings. Additionally, we found that the effect of changes was dose-dependent. These results indicated that genomic template stability (a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentration. Thus, DNA polymorphisms detected by RAPD analysis could be used as an investigation tool for environmental toxicology and as a useful biomarker assay for the detection of genotoxic effects of Cd pollution on plants. (C) 2005 Elsevier Ltd. All rights reserved. |
| 学科主题 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000232498700002 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55063]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Liu, W, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, W,Li, PJ,Qi, XM,et al. DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis[J]. CHEMOSPHERE,2005,61(2):158-167. |
| APA | Liu, W.,Li, PJ.,Qi, XM.,Zhou, Q.,Zheng, L.,...&Liu, W, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2005).DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis.CHEMOSPHERE,61(2),158-167. |
| MLA | Liu, W,et al."DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis".CHEMOSPHERE 61.2(2005):158-167. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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