A gas chromatographic/mass spectrometric method for tracing the microbial conversion of glucose into amino sugars in soil
文献类型：期刊论文
| 作者 | He, HB; Xie, HT; Zhang, XD; Wang, YH; Wu, YY; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terrestrial Ecol Proc, Shenyang 110016, Peoples R China |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2005 |
| 卷号 | 19期号:14页码:1993-1998 |
| ISSN号 | 0951-4198 |
| 英文摘要 | Amino sugars in soils are heterogeneous and have been used as microbial residue biomarkers to investigate the microbial contribution to soil organic matter. However, it is not clear what the available carbon source is and how glucose is utilized for the synthesis of soil amino sugars. This paper presents a new gas chromatography/mass spectrometry (GC/MS) approach for the identification of C-13 incorporation into three amino sugars, D-glucosamine, D-galactosamine, and muramic acid, in soil incubated with U-C-13-glucose. Method evaluation showed that the chemical ionization (CI) mode was suitable for all these amino sugars, but that electron impact (EI) mode was applicable only to glucosamine and galactosamine. The C-13 conversion rate was estimated based on the abundance ratio of the ions corresponding to the masses of the ions F + n and F (where n is the skeleton carbon number in the fragment ions F of the amino sugars) and calculated as atom percentage excess. The reproducibility of the method was excellent and clearly adequate for the present purpose. In addition, the new approach is highly accurate as tested with mixtures of U-C-13-glucose and natural glucose. Copyright (c) 2005 John Wiley & Sons, Ltd. |
| 学科主题 | Chemistry, Analytical ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000230666700012 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55103]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terrestrial Ecol Proc, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, HB,Xie, HT,Zhang, XD,et al. A gas chromatographic/mass spectrometric method for tracing the microbial conversion of glucose into amino sugars in soil[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2005,19(14):1993-1998. |
| APA | He, HB,Xie, HT,Zhang, XD,Wang, YH,Wu, YY,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terrestrial Ecol Proc, Shenyang 110016, Peoples R China.(2005).A gas chromatographic/mass spectrometric method for tracing the microbial conversion of glucose into amino sugars in soil.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,19(14),1993-1998. |
| MLA | He, HB,et al."A gas chromatographic/mass spectrometric method for tracing the microbial conversion of glucose into amino sugars in soil".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 19.14(2005):1993-1998. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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