Improvement of selectivity and cytotoxicity of IL-4 immunotoxin on lymphoma through site-directed mutagenesis
文献类型：期刊论文
| 作者 | Ji, JF; Cui, JX; Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Changchun 110016, Peoples R China; Lu, AG; Wu, WF |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:5页码:874-879 |
| 关键词 | Immunotoxin Interleukin-4(Il-4r) Pseudomonas Exotoxin a |
| ISSN号 | 0251-0790 |
| 英文摘要 | A wide variety of human cancer cells such as glioma and lymphoma express interleukin-4 receptors (IL-4R), therefore, it may be a good option to treat IL-4R-bearing tumor with IL-4-containing immunotoxins. By software analysis, two important amino acids 13T and 121R of IL-4 were chosen for site-directed mutation. Interleukin-A mutein cpIL-4(13D121E) was obtained through overlapping PCR and the chimeric immunotoxin was constructed by fusion of the gene encoding cpIL-4 (13D121E) to a gene encoding a modified Pseudomonas exotoxin A(PE38KDEL). The chimeric immunotoxin was expressed in E. coli with the yield of about 30% of the total bacterial protein. After being highly purified by affinity chromatography and anion exchange chromatography, the chimeric protein was tested for its cytotoxicity. The data show that cpIL-4(13D121E)-PE38KDEL had improved cytotoxicity to lymphoma cells Daudi expressing class I IL-4R in comparison with other IL-4-containing immunotoxin and had a lower cytotoxicity to endothelial cells expressing class II IL-4R. |
| 学科主题 | Chemistry, Multidisciplinary |
| 语种 | 中文 |
| WOS记录号 | WOS:000229296100022 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55139]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Changchun 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, JF,Cui, JX,Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Changchun 110016, Peoples R China,et al. Improvement of selectivity and cytotoxicity of IL-4 immunotoxin on lymphoma through site-directed mutagenesis[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2005,26(5):874-879. |
| APA | Ji, JF,Cui, JX,Wu, WF, Chinese Acad Sci, Shenyang Inst Appl Ecol, Changchun 110016, Peoples R China,Lu, AG,&Wu, WF.(2005).Improvement of selectivity and cytotoxicity of IL-4 immunotoxin on lymphoma through site-directed mutagenesis.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,26(5),874-879. |
| MLA | Ji, JF,et al."Improvement of selectivity and cytotoxicity of IL-4 immunotoxin on lymphoma through site-directed mutagenesis".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 26.5(2005):874-879. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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