Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity
文献类型：期刊论文
| 作者 | Cui, JX; Ji, JF; Lv, AG; Wu, WF; Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China |
| 刊名 | BIOCHEMISTRY-MOSCOW
 |
| 出版日期 | 2005 |
| 卷号 | 70期号:1页码:62-68 |
| 关键词 | Immunotoxin Interleukin-4 Pseudomonas Exotoxin a |
| ISSN号 | 0006-2979 |
| 英文摘要 | Interleukkin-4 receptors (IL-4Rs) are expressed on a wide variety of human cancer cells, and therefore it maybe a good option to treat IL-4R-bearing tumors with IL-4-fusing immunotoxins. In this study, the gene encoding human interleukin-4 mutein cpIL-4(13D) was obtained through overlapping polymerase chain reaction. A chimeric immunotoxin was constructed by genetically fusing the mutein cpIL-4(13D) to a modified version of Pseudomonas exotoxin A (PE38KDEL) and was expressed in Escherichia coli AD494 (DE3). The expression level of the fusion protein was about 30% of the total bacterial protein assessed by SDS-PAGE analysis. After purification by affinity chromatography and anion exchange chromatography, the chimeric protein was tested for its cytotoxicity. Our data show that cpIL-4(13D)-PE38KDEL has improved cytotoxicity on IL-4R-bearing tumor cells in comparison with other IL-4-fusing immunotoxins and might be useful in treating tumors with a large number of IL-4Rs. |
| 学科主题 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000227139600006 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55187]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, JX,Ji, JF,Lv, AG,et al. Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity[J]. BIOCHEMISTRY-MOSCOW,2005,70(1):62-68. |
| APA | Cui, JX,Ji, JF,Lv, AG,Wu, WF,&Wu, WF, Chinese Acad Sci, Inst Appl Ecol, 72,Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China.(2005).Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity.BIOCHEMISTRY-MOSCOW,70(1),62-68. |
| MLA | Cui, JX,et al."Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity".BIOCHEMISTRY-MOSCOW 70.1(2005):62-68. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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