Characterization of genetic diversity of Frankia strains in nodules of Alnus nepalensis (D. Don) from the Hengduan Mountains on the basis of PCR-RFLP analysis of the nifD-nifK IGS
文献类型：期刊论文
| 作者 | Dai, YM; He, XY; Zhang, CG; Zhang, ZZ; He, XY, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 刊名 | PLANT AND SOIL
 |
| 出版日期 | 2004 |
| 卷号 | 267期号:1-2页码:207-212 |
| 关键词 | Alnus Nepalensis Frankia Genetic Diversity Nifd-nifk Igs Pcr-rflp |
| ISSN号 | 0032-079X |
| 英文摘要 | Nodule samples from 90 A. nepalensis individuals were collected at five sites in the Hengduan Mountains. PCR-RFLP analysis of IGS between nifD and nifK genes was directly applied to uncultured Frankia strains in the nodules. Sizes of the nifD-nifK IGS amplicons and genetic distance between the RFLP patterns from these samples were noticeably different, indicating significant genetic variation in the Frankia population. There were some nodule samples, which produced more than one PCR fragment, and compound RFLP patterns, indicating that Frankia strains with different PCR-RFLP patterns coexisted in the same host plant under natural conditions. Among the 29 restriction patterns obtained, 5 patterns were found in more than one population and occurred in the majority of samples, while each of the other 24 patterns were represented by only one or two samples and were endemic to a particular population. From the calculated Gst and UPGMA cluster analysis. genetic diversity of Frankia strains was inferred to be related to climate and glaciation history in the Hengduan Mountains. |
| 学科主题 | Agronomy ; Plant Sciences ; Soil Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000226444500018 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55205]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | He, XY, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, YM,He, XY,Zhang, CG,et al. Characterization of genetic diversity of Frankia strains in nodules of Alnus nepalensis (D. Don) from the Hengduan Mountains on the basis of PCR-RFLP analysis of the nifD-nifK IGS[J]. PLANT AND SOIL,2004,267(1-2):207-212. |
| APA | Dai, YM,He, XY,Zhang, CG,Zhang, ZZ,&He, XY, Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China.(2004).Characterization of genetic diversity of Frankia strains in nodules of Alnus nepalensis (D. Don) from the Hengduan Mountains on the basis of PCR-RFLP analysis of the nifD-nifK IGS.PLANT AND SOIL,267(1-2),207-212. |
| MLA | Dai, YM,et al."Characterization of genetic diversity of Frankia strains in nodules of Alnus nepalensis (D. Don) from the Hengduan Mountains on the basis of PCR-RFLP analysis of the nifD-nifK IGS".PLANT AND SOIL 267.1-2(2004):207-212. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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