Site-directed mutagenesis of vascular endothelial growth factor (VEGF) by an improved overlap PCR
文献类型：期刊论文
| 作者 | Bai, XY; Lu, AG; Wu, WF; Sun, J; Niu, RF; Lu, AG, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2004 |
| 卷号 | 31期号:2页码:181-184 |
| 关键词 | Site-directed Mutagenesis Vascular Endothelial Grow Factor Overlap-pcr |
| ISSN号 | 1000-3282 |
| 英文摘要 | The PCR production of vascular endothelial growth factor (VEGF) was subcloned into a T-vector, and the recombined plasmids were then transformed into competent cell JM109. Eight white clones were selected randomly, and the recombined plasmids were extracted and mixed as template. Three selected primers were adapted and two rounds of PCR were performed to introduce mutations. The production of those were confirmed by enzyme digestion, PCR amplification and sequencing. The method is proved to be a simple, quick and easy way to introduce site-directed mutagenesis, and make latter work easy to do. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| WOS记录号 | WOS:000224104800016 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55253]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Lu, AG, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, XY,Lu, AG,Wu, WF,et al. Site-directed mutagenesis of vascular endothelial growth factor (VEGF) by an improved overlap PCR[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(2):181-184. |
| APA | Bai, XY,Lu, AG,Wu, WF,Sun, J,Niu, RF,&Lu, AG, Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang 110016, Peoples R China.(2004).Site-directed mutagenesis of vascular endothelial growth factor (VEGF) by an improved overlap PCR.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(2),181-184. |
| MLA | Bai, XY,et al."Site-directed mutagenesis of vascular endothelial growth factor (VEGF) by an improved overlap PCR".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.2(2004):181-184. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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