Crystallization and preliminary X-ray studies of methyl parathion hydrolase from Pseudomonas sp WBC-3
文献类型：期刊论文
| 作者 | Sun, L; Dong, YJ; Zhou, YF; Yang, MJ; Zhang, CG; Rao, ZH; Zhang, XE; Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| 出版日期 | 2004 |
| 卷号 | 60页码:954-956 |
| ISSN号 | 0907-4449 |
| 英文摘要 | Methyl parathion hydrolase (MPH) from Pseudomonas sp. WBC-3, an enzyme that catalyzes the degradation of methyl parathion (O,O-dimethyl O-p-nitrophenyl phosphorothioate; MP), has been purified and crystallized by the hanging-drop vapour-diffusion method. The crystals were grown at 291 K using a precipitant solution consisting of 30% PEG 400, 0.1 M sodium acetate pH 4.6, 0.1 M CdCl2. MPH is a zinc-containing enzyme judged by inductively coupled plasma mass-spectrometric (ICP-MS) analysis. Multiple-wavelength anomalous dispersive X-ray data were collected at 2.5 Angstrom resolution from a single crystal on beamline 41XU at SPring-8. The crystal belongs to space group P4(3)2(1)2, with unit-cell parameters a = 84.94, b = 84.94, c = 200.38 Angstrom, alpha = beta = gamma = 90degrees. The asymmetric unit contains two molecules and has a solvent content of similar to52%. Crystal structure determination is in progress. |
| 学科主题 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 语种 | 英语 |
| WOS记录号 | WOS:000220951000028 |
| 公开日期 | 2011-09-23 |
| 源URL | [http://210.72.129.5/handle/321005/55315]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所 |
| 通讯作者 | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, L,Dong, YJ,Zhou, YF,et al. Crystallization and preliminary X-ray studies of methyl parathion hydrolase from Pseudomonas sp WBC-3[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2004,60:954-956. |
| APA | Sun, L.,Dong, YJ.,Zhou, YF.,Yang, MJ.,Zhang, CG.,...&Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.(2004).Crystallization and preliminary X-ray studies of methyl parathion hydrolase from Pseudomonas sp WBC-3.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,60,954-956. |
| MLA | Sun, L,et al."Crystallization and preliminary X-ray studies of methyl parathion hydrolase from Pseudomonas sp WBC-3".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 60(2004):954-956. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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