A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles
文献类型：期刊论文
| 作者 | Zhang, Zhi1; Guan, Yueping1; Xu, Guoli1; Guo, Chen2 |
| 刊名 | MICROCHEMICAL JOURNAL
 |
| 出版日期 | 2019-06-01 |
| 卷号 | 147页码:824-831 |
| ISSN号 | 0026-265X |
| 关键词 | Washing-free Proximity Chemiluminescence Immunoassay Immunosensor Fe3O4 |
| DOI | 10.1016/j.microc.2019.03.064 |
| 英文摘要 | In this submission, a novel washing-free immunosensor was designed for chemiluminescence immunoassay (CLIA) of tumor marker using human Alpha-fetoprotein (AFP) as a representative. Reactive groups were introduced on the surface of the solvothermally prepared Fe3O4 magnetic submicron particles (MSPs) and the dual-functional polyethylene glycol (PEG) linkers were modified to reduce the non-specific adsorption capacity of the particle surface and further to immobilize N-(4-aminohexyl)-N-ethylisoluminol (AHEI) and anti-AFP antibody on the surface of the particles. After incubation with AFP and horseradish peroxidase (HRP) labeled anti-AFP antibody respectively, the formed sandwich-type immunocomplexes on the particle surface could lead to the close proximity between AHEI and HRP, thus eventually leads to an increase in the detected chemiluminescence (CL) signal. As a bound HRP labeled anti-AFP antibody produces much stronger CL signal than an unbound one, this approach eliminates the time- and labor-consuming washing steps usually required of such assays. Under the optimal conditions, this present method shows a wide quantitative range from 1 to 250 ng mL(-1) with a detection limit of 1.751 ng mL(-1). The detection limit was sufficient for measuring physiological (0-20 ng mL(-1)) and clinical ( > 20 ng mL(-1)) AFP levels. This method can also be applied in serum samples, and also avoid the problem of particle aggregation caused by multiple separation and washing steps. |
| WOS关键词 | ALPHA-FETOPROTEIN ; CHEMILUMINESCENCE DETECTION ; CANCER STATISTICS ; IMMUNOASSAY ; SERUM ; NANOPARTICLES ; BINDING ; ASSAY ; DNA |
| 资助项目 | National Key Research and Development Program of China[2018YFF0214900] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000468714400100 |
| 资助机构 | National Key Research and Development Program of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/29791]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Guan, Yueping |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhi,Guan, Yueping,Xu, Guoli,et al. A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles[J]. MICROCHEMICAL JOURNAL,2019,147:824-831. |
| APA | Zhang, Zhi,Guan, Yueping,Xu, Guoli,&Guo, Chen.(2019).A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles.MICROCHEMICAL JOURNAL,147,824-831. |
| MLA | Zhang, Zhi,et al."A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles".MICROCHEMICAL JOURNAL 147(2019):824-831. |
入库方式： OAI收割
来源：过程工程研究所
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