Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus
文献类型：期刊论文
| 作者 | Sharshar, Moustafa Mohamed1,2; Samak, Nadia Abdrabo1,2,3; Hao, Xuemi1,2; Mu, Tingzhen1; Zhong, Wei1,2; Yang, Maohua1; Peh, Sumit1,2; Ambreen, Sadaf2,4; Xing, Jianmin1,2
|
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2019-09-01 |
| 卷号 | 288页码:9 |
| 关键词 | Thioalkalivibrio versutus Fed-batch cultivation Bio-desulfurization Inducible regulation system Promoter engineering |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2019.121486 |
| 英文摘要 | Highly toxic and flammable H2S gas has become an environmental threat. Because of its ability to efficiently remove H2S by oxidation, Thioalkalivibrio versutus is gaining more attention. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow weakly. Weak growth makes any trial for developing potent genetic tools required for genetic engineering far from achieved. In this study, the fed-batch strategy improved T. versutus growth by 1.6 fold in maximal growth rate, 9-fold in 0.D-600 values and about 3-fold in biomass and protein productions. The strategy also increased the favorable desulfurization product, sulfur, by 2.7 fold in percent yield and 1.5-fold in diameter. A tight iron-inducible expression system for T. versutus was successfully developed. The system was derived from fed-batch cultivation coupled with new design, build, test and validate (DPTV) approach. The inducible system was validated by toxin expression. Fed-batch cultivation coupled with DPTV approach could be applied to other autotrophs. |
| WOS关键词 | FED-BATCH CULTIVATION ; ESCHERICHIA-COLI ; SYNTHETIC BIOLOGY ; GENE-EXPRESSION ; PERFORMANCE ; VECTORS ; SULFIDE ; IRON ; TRANSLATION ; PROTEINS |
| 资助项目 | National Science Foundation of China[21878307] ; National Science Foundation of China[31800030] ; National Science Foundation of China[31872633] ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000472018100041 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Science Foundation of China ; CAS-TWAS (The World Academy of Sciences) President's Fellowship for International PhD students |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/29955]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Xing, Jianmin |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt 4.Chinese Acad Sci, Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabo,Hao, Xuemi,et al. Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2019,288:9. |
| APA | Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabo.,Hao, Xuemi.,Mu, Tingzhen.,Zhong, Wei.,...&Xing, Jianmin.(2019).Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,288,9. |
| MLA | Sharshar, Moustafa Mohamed,et al."Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 288(2019):9. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。