Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation
文献类型：期刊论文
| 作者 | Liaw, Bor-Shuang1; Xing, Fangyu2; Wang, Dan1; Gao, Fei3; Lu, Jingsong4; Yu, Jing4; Sun, Xiaodan1; Wang, Xiumei1; Feng, Qingling1; Zhang, Guifeng2 |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 179页码:48-55 |
| 关键词 | Collagen fibrillogenesis Langmuir-Blodgett deposition Self-assembly Cell adhesion Cell proliferation |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2019.03.053 |
| 英文摘要 | Collagen fibrillogenesis is of special significance for the maintenance of collagen scaffold's mechanical stability and biological performance. Comprehensive information about the mechanism of collagen fibrillogenesis in vitro, as well as the effect of fibrillogenesis on deposited layers of ordered collagen molecules for cellular behavior regulation is thus crucial. In the current study, the pH, phosphate ion as well as reconstitution time impacting on the in vitro fibrillogenesis was systematically investigated, including the zeta potential and turbidity measurement. Furthermore, the fibrillogenesis impacting on the pi-a isotherms of collagen assembly at the air/water interface was then fully evaluated. By applying LB technique, collagen fibril-assembling arrays structure can be successfully transferred to form surface deposition onto the mica and glass substrate. The morphology and collagen content were subsequently assessed by atomic force microscopy (AFM) and hydrolyzing examination respectively. Effect of collagen LB deposition on the adhesion and proliferation of SD rat bone marrow mesenchymal stem cells were estimated by Rhodamine Phalloidin/DIPI staining and CCK8 proliferation assays. The results show that highly oriented and collagen-abundant thin film can further facilitate cell adhesion and proliferation, indicating an innovative direction for tissue engineering. |
| WOS关键词 | MOLECULAR-INTERACTIONS ; SCHAEFER FILMS ; PACLITAXEL ; ADSORPTION ; MICROSCOPY ; NETWORK ; PH |
| 资助项目 | Intergovernmental cooperation in science and technology[2016YFE0125300] ; National Natural Science Foundation of China (NSFC)[81671829] ; National Key R&D Program of China[2018YFA0108202] ; National Key R&D Program of China[2018YFC1106402] ; Science and Technology Program of Guangzhou, China[201803010086] |
| WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000471736600006 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | Intergovernmental cooperation in science and technology ; National Natural Science Foundation of China (NSFC) ; National Key R&D Program of China ; Science and Technology Program of Guangzhou, China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/30035]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Guifeng; Zhao, Lingyun |
| 作者单位 | 1.Tsinghua Univ, Sch Mat Sci & Engn, State Key Lab New Ceram & Fine Proc, Key Lab Adv Mat,Minist Educ China, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Northwest Univ, Coll Chem & Mat Sci, Xian 710069, Shaanxi, Peoples R China 4.Zhejiang Univ Technol, Coll Mat Sci & Engn, Hangzhou 310014, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liaw, Bor-Shuang,Xing, Fangyu,Wang, Dan,et al. Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,179:48-55. |
| APA | Liaw, Bor-Shuang.,Xing, Fangyu.,Wang, Dan.,Gao, Fei.,Lu, Jingsong.,...&Zhao, Lingyun.(2019).Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation.COLLOIDS AND SURFACES B-BIOINTERFACES,179,48-55. |
| MLA | Liaw, Bor-Shuang,et al."Effect of in vitro collagen fibrillogenesis on Langmuir-Blodgett (LB) deposition for cellular behavior regulation".COLLOIDS AND SURFACES B-BIOINTERFACES 179(2019):48-55. |
入库方式： OAI收割
来源：过程工程研究所
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