Development of an immunochromatographic strip test for rapid detection of lily symptomless virus
文献类型：期刊论文
| 作者 | Zhang, Yubao1; Wang, Yajun1; Meng, Jing2; Xie, Zhongkui1; Wang, Ruoyu1; Kutcher, Hadley Randal3; Guo, Zhihong1 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 220页码:13-17 |
| 关键词 | Immunonochromatographic strip Lsv Pcr Rapid detection |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2015.03.021 |
| 通讯作者 | Xie, zhongkui(wxhcas@lzb.ac.cn) |
| 英文摘要 | A rapid immunochromatographic strip (ics) test for specific detection of lily symptomless virus (lsv) was developed. the test is based on a double-antibody sandwich format and employs two distinct anti-lsv polyclonal antibodies (igg(1) and igg(2)). the first antibody, igg(1) was used as the detection antibody conjugated to colloidal gold and the second antibody, igg(2) was used to as the capture antibody at the test line. the performance of the ics test was evaluated and the results obtained were compared with a quadruplex rt-pcr assay. when serial dilutions of purified lsv were tested, the lsv detection limit of the ics test was 6.0 x 10(-8) mg/ml, which was the same as the quadruplex rt-pcr assay. relative to quadruplex rt-pcr, the specificity and sensitivity of the ics were 98.6% and 100%, respectively for field leaf samples. there was significant agreement between the results of the ics and quadruplex rt-pcr tests (kappa = 0.983). compared with conventional lily virus detection methods, our ics test has many advantages: simple, fast, low cost, high sensitivity and specificity, and has applications in the laboratory and in the field to detect and control lsv. (c) 2015 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | RT-PCR ; MOTTLE VIRUS ; LILIUM ; PURIFICATION ; SEROTYPE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000356112300003 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555027 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Xie, Zhongkui |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Ningxia Agr Comprehens Dev Off, Yinchuan 750002, Peoples R China 3.Univ Saskatchewan, Coll Agr & Bioresources, Saskatoon, SK S7N 5A8, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Yubao,Wang, Yajun,Meng, Jing,et al. Development of an immunochromatographic strip test for rapid detection of lily symptomless virus[J]. Journal of virological methods,2015,220:13-17. |
| APA | Zhang, Yubao.,Wang, Yajun.,Meng, Jing.,Xie, Zhongkui.,Wang, Ruoyu.,...&Guo, Zhihong.(2015).Development of an immunochromatographic strip test for rapid detection of lily symptomless virus.Journal of virological methods,220,13-17. |
| MLA | Zhang, Yubao,et al."Development of an immunochromatographic strip test for rapid detection of lily symptomless virus".Journal of virological methods 220(2015):13-17. |
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来源：寒区旱区环境与工程研究所
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