microRNA-16-5p enhances radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway in prostate cancer cells
文献类型：期刊论文
| 作者 | Wang, Fang1,4,5,6; Mao, Aihong2; Tang, Jinzhou3; Zhang, Qianjing1,4,5,6; Yan, Junfang1,4,5,6; Wang, Yupei1,4,5,6; Di, Cuixia1,4,5; Gan, Lu1,4,5,6; Sun, Chao1,4,5; Zhang, Hong1,4,5,7 |
| 刊名 | JOURNAL OF CELLULAR PHYSIOLOGY
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| 出版日期 | 2019-08-01 |
| 卷号 | 234期号:8页码:13182-13190 |
| ISSN号 | 0021-9541 |
| 关键词 | cell cycle arrest miR-16-5p prostate cancer radiosensitivity |
| DOI | 10.1002/jcp.27989 |
| 通讯作者 | Zhang, Hong(zhangh@impcas.ac.cn) |
| 英文摘要 | Prostate cancer (CaP) is the second most common cancer in men worldwide in 2012, and radiation therapy is one of the most common definitive treatment options for localized CaP. However, radioresistance is a major challenge for the current radiotherapy, accumulating evidences suggest microRNAs (miRNAs), as an important regulator in cellular ionizing radiation (IR) responses, are closely correlated with radiosensitivity in many cancers. Here, we identified microRNA-16-5p(miR-16-5p) is significantly upregulated in CaP LNCaP cells following IR and can enhance radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway. To identify the expression profile of miRNAs in CaP cells exposed to IR, we performed human miRNA probe hybridization chip analysis and miR-16-5p was found to be significantly overexpressed in all treatment groups that irradiated with different doses of X-rays and heavy ions (C-12(6+)). Furthermore, overexpression of miR-16-5p suppressed cell proliferation, reduced cell viability, and induced cell cycle arrest at G0/G1 phase, resulting in enhanced radiosensitivity in LNCaP cells. Additionally, miR-16-5p specifically targeted the Cyclin D1/E1-3-UTR in LNCaP cells and affected the expression of Cyclin D1/E1 in both mRNA and protein levels. Taken together, miR-16-5p enhanced radiosensitivity ofCaP cells, the mechanism may be through modulating Cyclin D1/Cyclin E1/pRb/E2F1 pathway to cause cell cycle arrest at G0/G1 phase. These findings provided new insight into the correlation between miR-16-5p, cell cycle arrest, and radiosensitivity in CaP, revealed a previously unrecognized function of miR-16-5p-Cyclin D1/E1-pRb-E2F1 regulation in response to IR and may offer an alternative therapy to improve the efficiency of conventional radiotherapy. |
| 收录类别 | SCI |
| WOS关键词 | DEPENDENT KINASE INHIBITOR ; MICRO-RNA ; CARCINOMA ; RADIOTHERAPY ; BIOGENESIS ; MIR-15A ; RADIORESISTANCE ; SENSITIVITY ; STATISTICS ; GROWTH |
| WOS研究方向 | Cell Biology ; Physiology |
| WOS类目 | Cell Biology ; Physiology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000467240800093 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555452 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 2.Inst Gansu Med Sci Res, Lanzhou, Gansu, Peoples R China 3.Lanzhou Univ, Sch Life Sci, Lanzhou, Gansu, Peoples R China 4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med, Lanzhou, Gansu, Peoples R China 5.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou, Gansu, Peoples R China 6.Univ Chinese Acad Sci, Sch Life Sci, Beijing, Peoples R China 7.Gansu Wuwei Tumor Hosp, Wuwei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fang,Mao, Aihong,Tang, Jinzhou,et al. microRNA-16-5p enhances radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway in prostate cancer cells[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(8):13182-13190. |
| APA | Wang, Fang.,Mao, Aihong.,Tang, Jinzhou.,Zhang, Qianjing.,Yan, Junfang.,...&Zhang, Hong.(2019).microRNA-16-5p enhances radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway in prostate cancer cells.JOURNAL OF CELLULAR PHYSIOLOGY,234(8),13182-13190. |
| MLA | Wang, Fang,et al."microRNA-16-5p enhances radiosensitivity through modulating Cyclin D1/E1-pRb-E2F1 pathway in prostate cancer cells".JOURNAL OF CELLULAR PHYSIOLOGY 234.8(2019):13182-13190. |
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来源：寒区旱区环境与工程研究所
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