Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins
文献类型：期刊论文
| 作者 | Qiao, Jianyu1,2,3; Gao, Ruibin4; Wang, Zhongyang1,2,3; Sun, Yaming1,2,3; Dong, Shuqing1,2; Zhao, Liang1,2 |
| 刊名 | EMIRATES JOURNAL OF FOOD AND AGRICULTURE
 |
| 出版日期 | 2019-03-01 |
| 卷号 | 31期号:3页码:164-171 |
| 关键词 | Capillary electrophoresis Amino acids Derivatization Jujube |
| ISSN号 | 2079-052X |
| DOI | 10.9755/ejfa.2019.v31.i3.1928 |
| 通讯作者 | Dong, Shuqing(sqdong@licp.cas.cn) ; Zhao, Liang(zhaol@licp.cas.cn) |
| 英文摘要 | A simple capillary electrophoresis (CE) method that using beta-cyclodextrin (beta-CD) as buffer modifier has been developed for the detection of ten amino acids of jujube from five different geographical origins. The CE methodology was optimized through the variation of type, pH and concentration of the buffer solution, concentration of the additive beta-CD, applied voltage, injection time and wavelength of the UV detection. It was found that the best separation of ten kinds of amino acids was achieved within less than 6 min under the optimum separation conditions: 50 mmol L-1 pH 9.5 borate-phosphate buffer with 5.0 mmol L-1 beta-CD, 15 kV applied voltage, 25 degrees C column temperature, 210 nm detection wavelength, and 5 s injection time. This method showed good repeatability with RSD values of 1.4-3.4% for peak area, and 1.4-4.7% for migration time, when beta-CD was used as buffer modifier. Under the optimum conditions, the method has been successfully applied to the detection of actual jujube samples, which also verifies the effectiveness and practicability of the method. Recovery of real samples was in ranging of 90%-105%, which proved the feasibility of the method. It also proved that the method was successfully applied to the quantitative analysis of amino acids of interest in plant samples. |
| 收录类别 | SCI |
| WOS关键词 | ZIZIPHUS-JUJUBA ; ZIZYPHUS-JUJUBA ; POLYSACCHARIDES ; FLAVONOIDS ; COMPLEXES ; SAPONINS ; FRUITS |
| WOS研究方向 | Agriculture ; Food Science & Technology |
| WOS类目 | Agronomy ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000468204100003 |
| 出版者 | UNITED ARAB EMIRATES UNIV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555460 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Dong, Shuqing; Zhao, Liang |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China 4.Third Prov Peoples Hosp Gansu, Dept Pharm, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Jianyu,Gao, Ruibin,Wang, Zhongyang,et al. Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins[J]. EMIRATES JOURNAL OF FOOD AND AGRICULTURE,2019,31(3):164-171. |
| APA | Qiao, Jianyu,Gao, Ruibin,Wang, Zhongyang,Sun, Yaming,Dong, Shuqing,&Zhao, Liang.(2019).Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins.EMIRATES JOURNAL OF FOOD AND AGRICULTURE,31(3),164-171. |
| MLA | Qiao, Jianyu,et al."Application of capillary electrophoresis for assessing amino acids composition of Jujube from different geographical origins".EMIRATES JOURNAL OF FOOD AND AGRICULTURE 31.3(2019):164-171. |
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来源：寒区旱区环境与工程研究所
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