Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells
文献类型：期刊论文
| 作者 | Zhang, Qianjing1,2,3; Di, Cuixia1,2,3; Yan, Junfang1,2,3; Wang, Fang1,2,3; Qu, Tao4; Wang, Yupei1,2,3; Chen, Yuhong1,2,3; Zhang, Xuetian1,2,3; Liu, Yang1,2,3; Yang, Hongying5 |
| 刊名 | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 47期号:1页码:1273-1280 |
| ISSN号 | 2169-1401 |
| 关键词 | Alternative splicing apoptosis cancer pladienolide B p73 SF3b1 |
| DOI | 10.1080/21691401.2019.1596922 |
| 通讯作者 | Zhang, Hong(zhang.h@impcas.ac.cn) |
| 英文摘要 | Pladienolide B is a potent cancer cell growth inhibitor that targets the SF3b1 subunit of the spliceosome. There is considerable interest in the compound as a tool to study SF3b1 function in cancer. However, so far little information is available on the molecular mechanism of SF3b1 eliciting apoptosis in cancer cells. Here, we investigated the molecular mechanism of SF3b1 eliciting apoptosis in human cervical carcinoma cells. We demonstrated that inhibition of SF3b1 by pladienolide B inhibited proliferation of HeLa cells at low nanomolar concentrations in a dose- and time-dependent manner. It also induced G2/M phase arrest and significant rise of apoptotic cells. Moreover, it is indicated that inhibition of SF3b1 by pladienolide B induced Tap73/Delta Np73 expression and consequently down-regulated Bax/Bcl-2 ratio, cytochrome c release and caspase-3 expression. Thus, our results showed that SF3b1 plays a pivotal role in cycle arrest, apoptosis induction, and p73 splicing in human cervical carcinoma cells, suggesting that SF3b1 could be used as a potential candidate for cervical cancer therapy. |
| 收录类别 | SCI |
| WOS关键词 | IN-VITRO ; TARGET ; CANCER ; PATHWAY ; TAP73/DELTA-NP73 ; SPLICEOSOME ; PROGNOSIS ; PROTEINS ; RATIO ; LIFE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| WOS类目 | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:000463887200001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555491 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Sch Life Sci, Beijing, Peoples R China 4.Gansu Prov Hosp, Dept Biotherapy Ctr, Lanzhou, Gansu, Peoples R China 5.Soochow Univ, Med Coll Soochow, Med Sch Radiat Med & Protect, Suzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qianjing,Di, Cuixia,Yan, Junfang,et al. Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells[J]. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,2019,47(1):1273-1280. |
| APA | Zhang, Qianjing.,Di, Cuixia.,Yan, Junfang.,Wang, Fang.,Qu, Tao.,...&Zhang, Hong.(2019).Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,47(1),1273-1280. |
| MLA | Zhang, Qianjing,et al."Inhibition of SF3b1 by pladienolide B evokes cycle arrest, apoptosis induction and p73 splicing in human cervical carcinoma cells".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 47.1(2019):1273-1280. |
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来源：寒区旱区环境与工程研究所
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