Effects of the extract of Ammopiptanthus mongolicus cheng f.(JA1) on induction of apoptosis of HepG2 in vitro and its molecular mechanisms
文献类型：期刊论文
| 作者 | Jia, L; An, LZ |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 19期号:2页码:118-123 |
| 关键词 | extract from Aminopiptanthus mongolicus cheng f. (JA1) HepG2 apoptosis induction wt-p53 |
| ISSN号 | 0895-3988 |
| 通讯作者 | An, LZ() |
| 英文摘要 | Objective To study the effects and the mechanisms of extract from a leguminous plant (Ammopiptanthus mongolicus cheng f.) (JA1) in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro. Methods The HepG2 cell line was used as target cells. The effect of JA1 on HepG2 cell growth was detected by microculture tetrazolium assay (MTT), flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. Results JA1 not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JA1-treated HepG2 cells under transmission electronic microscope, "Sub-G1" phase peak occurred in flow cytometry and DNA "ladder" was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and JA1-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased. Conclusions JA1 could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JA1 can be used as a good source of medicinal plant for the treatment of hepatocarcinoma. |
| 收录类别 | SCI |
| WOS关键词 | CELLS |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000237238500006 |
| 出版者 | CHINESE ACAD PREVENTIVE MEDICINE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555759 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, LZ |
| 作者单位 | 1.Lanzhou Univ, Minist Educ, Key Lab Arid & Pastoral Agroecol, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Pastoral Agroecol, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Key Lab Desert & Desertificat, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, L,An, LZ. Effects of the extract of Ammopiptanthus mongolicus cheng f.(JA1) on induction of apoptosis of HepG2 in vitro and its molecular mechanisms[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2006,19(2):118-123. |
| APA | Jia, L,&An, LZ.(2006).Effects of the extract of Ammopiptanthus mongolicus cheng f.(JA1) on induction of apoptosis of HepG2 in vitro and its molecular mechanisms.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,19(2),118-123. |
| MLA | Jia, L,et al."Effects of the extract of Ammopiptanthus mongolicus cheng f.(JA1) on induction of apoptosis of HepG2 in vitro and its molecular mechanisms".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 19.2(2006):118-123. |
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来源：寒区旱区环境与工程研究所
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