Plant regeneration of Chorispora bungeana via somatic embryogenesis
文献类型：期刊论文
| 作者 | Wang, JH; An, LZ; Yang, DQ; Si, J; Fu, XY; Chang, JF; Xu, SJ |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 42期号:2页码:148-151 |
| 关键词 | chromosome number somatic embryo sucrose concentration suspension cell culture tissue culture |
| ISSN号 | 1054-5476 |
| DOI | 10.1079/IVP2006718 |
| 通讯作者 | An, LZ() |
| 英文摘要 | A method was developed for in vitro regeneration of plants via somatic embryogenesis in Chorispora bungeana, an alpine plant with freeze-tolerance, using cell suspensions initiated from leaf-derived callus. Primary calli were induced from leaves of C. bungeana grown on Murashige and Skoog (MS) media supplemented with 4.0 mg l(-1) gibberellic acid (GA(3)), 0.2 mg l(-1) alpha-naphthaleneacetic acid (NAA) and 0.2 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Suspension culture was initiated by incubating the callus particulates in liquid MS medium supplemented with 1.0 mg l(-1) kinetin (KT) and 0.2 mg l(-1) NAA. Individual early cotyledonary-stage somatic embryos isolated from cell suspension developed into whole plants on medium containing high levels of sucrose (60 and 90 g l(-1)), whereas lower sucrose concentrations (0 and 30 g l(-1)) were inhibitory to main root development. On the MS medium with 90 g l(-1) sucrose, one regenerated plant exhibited hetero-morphologic leaves, while other plants grown on different media showed a transformation from stem to root. |
| 收录类别 | SCI |
| WOS关键词 | IN-VITRO ; GROWTH-REGULATORS ; CO2 ENRICHMENT ; SUCROSE ; CULTURE ; L. ; NITROGEN |
| WOS研究方向 | Plant Sciences ; Cell Biology ; Developmental Biology |
| WOS类目 | Plant Sciences ; Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000237232600011 |
| 出版者 | CABI PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555762 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, LZ |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, JH,An, LZ,Yang, DQ,et al. Plant regeneration of Chorispora bungeana via somatic embryogenesis[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2006,42(2):148-151. |
| APA | Wang, JH.,An, LZ.,Yang, DQ.,Si, J.,Fu, XY.,...&Xu, SJ.(2006).Plant regeneration of Chorispora bungeana via somatic embryogenesis.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,42(2),148-151. |
| MLA | Wang, JH,et al."Plant regeneration of Chorispora bungeana via somatic embryogenesis".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 42.2(2006):148-151. |
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来源：寒区旱区环境与工程研究所
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