Phylogenetic diversity of culturable bacteria from alpine permafrost in the Tianshan Mountains, northwestern China
文献类型：期刊论文
| 作者 | Bai, Yu; Yang, Daqun; Wang, Hanhui; Xu, Shijian; Wang, Xiaoxiang; An, Lizhe |
| 刊名 | RESEARCH IN MICROBIOLOGY
 |
| 出版日期 | 2006-10-01 |
| 卷号 | 157期号:8页码:741-751 |
| 关键词 | alpine permafrost bacterial diversity 16S rDNA |
| ISSN号 | 0923-2508 |
| DOI | 10.1016/j.resmic.2006.03.006 |
| 通讯作者 | An, Lizhe(lizhean@lzu.edu.cn) |
| 英文摘要 | Microbes have been discovered in permafrost sediments for nearly a century. However, microbiological analyses of alpine permafrost are very scarce. This study is a first attempt to describe the phylogenetic diversity of a culturable bacterial community isolated from alpine permafrost in the Tianshan Mountains in northwestern China. Aerobic 2.5-6.0 x 10(5) CFU/gdw (CFU per 1 gram of dry weight) on modified PYGV medium were recovered from alpine permafrost samples at 4 degrees C; among these, 91 bacterial isolates with different morphotypes were characterized by phenotypic properties, such as morphology, colony pigmentation, Gram staining, endospore formation and temperature range of growth. The isolates were further categorized based on amplified rDNA restriction analysis (ARDRA), and 51 representative isolates possessing distinct ARDRA patterns selected for subsequent 16S rDNA sequencing and phylogenetic analysis. The phylogenetic trees placed the 51 isolates in four major groups: the high-G + C Gram-positives, the low-G + C Gram-positives, Proteobacteria and the Cytophaga-Flavobacterium-Bacteroides (CFB) phylum. The most abundant and diverse isolates were members of Gram-positive bacteria, particularly the Arthrobacter as a dominant group in alpine permafrost culturable populations. Results of the Jukes-Cantor evolutionary distance matrix suggested that the vast majority of the isolates were different strains of known species, and three may represent new species within the genus Chryseobacterium of the CFB phylum. From this study, it is proposed that alpine permafrost sediments in the Tianshan Mountains provide a specific ecological niche for prolonging survival of diverse microbial lineages. (c) 2006 Elsevier SAS. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | PSYCHROPHILE PSEUDOALTEROMONAS-HALOPLANKTIS ; SIBERIAN PERMAFROST ; BETA-GALACTOSIDASE ; RIBOSOMAL-RNA ; SEA-ICE ; MICROORGANISMS ; TEMPERATURE ; SEQUENCES ; GENES ; CORE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000241079300006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555792 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Agroecol, Minist Educ, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Regions Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yu,Yang, Daqun,Wang, Hanhui,et al. Phylogenetic diversity of culturable bacteria from alpine permafrost in the Tianshan Mountains, northwestern China[J]. RESEARCH IN MICROBIOLOGY,2006,157(8):741-751. |
| APA | Bai, Yu,Yang, Daqun,Wang, Hanhui,Xu, Shijian,Wang, Xiaoxiang,&An, Lizhe.(2006).Phylogenetic diversity of culturable bacteria from alpine permafrost in the Tianshan Mountains, northwestern China.RESEARCH IN MICROBIOLOGY,157(8),741-751. |
| MLA | Bai, Yu,et al."Phylogenetic diversity of culturable bacteria from alpine permafrost in the Tianshan Mountains, northwestern China".RESEARCH IN MICROBIOLOGY 157.8(2006):741-751. |
入库方式： iSwitch采集
来源：寒区旱区环境与工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。