Reaction of atrazine with protein and its application to detection of protein
文献类型:期刊论文
作者 | Shi, DR; Zhang, T; Ren, LP; Yu, JL; Zhang, CR; Rao, ZH |
刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
![]() |
出版日期 | 2006-03-01 |
卷号 | 26期号:3页码:509-512 |
关键词 | atrazine protein interaction RLS |
ISSN号 | 1000-0593 |
通讯作者 | Ren, LP() |
英文摘要 | The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin (BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O-H-...pi hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 mu g(.)mL(-1). Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3 sigma) is 12 ng (.)mL(.)(-1) Synthesis samples were determined with satisfactory results. |
收录类别 | SCI |
WOS关键词 | LIGHT-SCATTERING TECHNIQUE |
WOS研究方向 | Spectroscopy |
WOS类目 | Spectroscopy |
语种 | 英语 |
WOS记录号 | WOS:000236364900032 |
出版者 | BEIJING UNIV PRESS |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555846 |
专题 | 寒区旱区环境与工程研究所 |
通讯作者 | Ren, LP |
作者单位 | 1.China Agr Univ, Coll Sci, Dept Appl Chem, Beijing 100094, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Shanghai Normal Univ, Coll Tourism, Dept Environm Sci & Engn, Shanghai 200234, Peoples R China |
推荐引用方式 GB/T 7714 | Shi, DR,Zhang, T,Ren, LP,et al. Reaction of atrazine with protein and its application to detection of protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2006,26(3):509-512. |
APA | Shi, DR,Zhang, T,Ren, LP,Yu, JL,Zhang, CR,&Rao, ZH.(2006).Reaction of atrazine with protein and its application to detection of protein.SPECTROSCOPY AND SPECTRAL ANALYSIS,26(3),509-512. |
MLA | Shi, DR,et al."Reaction of atrazine with protein and its application to detection of protein".SPECTROSCOPY AND SPECTRAL ANALYSIS 26.3(2006):509-512. |
入库方式: iSwitch采集
来源:寒区旱区环境与工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。