中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Reaction of atrazine with protein and its application to detection of protein

文献类型:期刊论文

作者Shi, DR; Zhang, T; Ren, LP; Yu, JL; Zhang, CR; Rao, ZH
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
出版日期2006-03-01
卷号26期号:3页码:509-512
关键词atrazine protein interaction RLS
ISSN号1000-0593
通讯作者Ren, LP()
英文摘要The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin (BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O-H-...pi hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 mu g(.)mL(-1). Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3 sigma) is 12 ng (.)mL(.)(-1) Synthesis samples were determined with satisfactory results.
收录类别SCI
WOS关键词LIGHT-SCATTERING TECHNIQUE
WOS研究方向Spectroscopy
WOS类目Spectroscopy
语种英语
WOS记录号WOS:000236364900032
出版者BEIJING UNIV PRESS
URI标识http://www.irgrid.ac.cn/handle/1471x/2555846
专题寒区旱区环境与工程研究所
通讯作者Ren, LP
作者单位1.China Agr Univ, Coll Sci, Dept Appl Chem, Beijing 100094, Peoples R China
2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China
3.Shanghai Normal Univ, Coll Tourism, Dept Environm Sci & Engn, Shanghai 200234, Peoples R China
推荐引用方式
GB/T 7714
Shi, DR,Zhang, T,Ren, LP,et al. Reaction of atrazine with protein and its application to detection of protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2006,26(3):509-512.
APA Shi, DR,Zhang, T,Ren, LP,Yu, JL,Zhang, CR,&Rao, ZH.(2006).Reaction of atrazine with protein and its application to detection of protein.SPECTROSCOPY AND SPECTRAL ANALYSIS,26(3),509-512.
MLA Shi, DR,et al."Reaction of atrazine with protein and its application to detection of protein".SPECTROSCOPY AND SPECTRAL ANALYSIS 26.3(2006):509-512.

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来源:寒区旱区环境与工程研究所

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