Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica
文献类型：期刊论文
| 作者 | Wu, Yuxia; Ding, Nan; Zhao, Xin; Zhao, Mingui; Chang, Zongqiang; Liu, Jianquan; Zhang, Lixin |
| 刊名 | PLANT MOLECULAR BIOLOGY
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 65期号:1-2页码:1-11 |
| 关键词 | E. coli mutant complementation Na+/H+ antiporter Populus euphratica salt tolerance |
| ISSN号 | 0167-4412 |
| DOI | 10.1007/s11103-007-9170-y |
| 通讯作者 | Zhang, Lixin(zhanglixin@ibcas.ac.cn) |
| 英文摘要 | Populus euphratica is a salt-tolerant tree species growing in semi-arid saline areas. A Na+/H+ antiporter gene was successfully isolated from this species through RACE cloning, and named PeSOS1. The isolated cDNA was 3665 bp long and contained a 3438 bp open reading frame that was predicted to encode a 127-kDa protein with 12 hypothetical transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail in the C-terminal part. The amino acid sequence of this PeSOS1 gene showed 64% identity with the previously isolated SOS1 gene from the glycophyte Arabidopsis thaliana. The level of protein expressed by PeSOS1 in the leaves of P. euphratica was significantly up-regulated in the presence of high (200 mM) concentrations of NaCl, while the mRNA level in the leaves remained relatively constant. Immunoanalysis suggested that the protein encoded by PeSOS1 is localized in the plasma membrane. Expression of PeSOS1 partially suppressed the salt sensitive phenotypes of the EP432 bacterial strain, which lacks the activity of the two Na+/H+ antiporters EcNhaA and EcNhaB. These results suggest that PeSOS1 may play an essential role in the salt tolerance of P. euphratica and may be useful for improving salt tolerance in other tree species. |
| 收录类别 | SCI |
| WOS关键词 | MEMBRANE H+-ATPASE ; PLASMA-MEMBRANE ; ESCHERICHIA-COLI ; SALT TOLERANCE ; ARABIDOPSIS-THALIANA ; SACCHAROMYCES-CEREVISIAE ; ATRIPLEX GMELINI ; STRESS TOLERANCE ; SODIUM-TRANSPORT ; PLANT-RESPONSES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000248815400001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555899 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, Lixin |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, MOE Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yuxia,Ding, Nan,Zhao, Xin,et al. Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica[J]. PLANT MOLECULAR BIOLOGY,2007,65(1-2):1-11. |
| APA | Wu, Yuxia.,Ding, Nan.,Zhao, Xin.,Zhao, Mingui.,Chang, Zongqiang.,...&Zhang, Lixin.(2007).Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica.PLANT MOLECULAR BIOLOGY,65(1-2),1-11. |
| MLA | Wu, Yuxia,et al."Molecular characterization of PeSOS1: the putative Na+/H+ antiporter of Populus euphratica".PLANT MOLECULAR BIOLOGY 65.1-2(2007):1-11. |
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来源：寒区旱区环境与工程研究所
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