NADPH oxidase-dependent hydrogen peroxide production, induced by salinity stress, may be involved in the regulation of total calcium in roots of wheat
文献类型：期刊论文
| 作者 | Yang, Yingli; Xua, Shijian; An, Lizhe; Chen, Nianlai |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 164期号:11页码:1429-1435 |
| 关键词 | H2O2 NADPH oxidase salt stress total Ca content wheat |
| ISSN号 | 0176-1617 |
| DOI | 10.1016/j.jplph.2006.08.009 |
| 通讯作者 | An, Lizhe(lizhean@lzu.edu.cn) |
| 英文摘要 | Hydrogen peroxide (H2O2) is often generated by cells and tissues under environmental stress. In this work, we provide evidence that plasma membrane (PM) NADPH oxidase-dependent H2O2 production might act as an intermediate step in the NaCl-induced elevation of calcium (Ca) in roots of wheat. Remarkable increases in the content of total Ca were observed not only in roots exposed to NaCl but also in roots of seedlings exposed to exogenous H2O2. In roots, H2O2 production increased upon exposure to salt stress. PM vesicles were isolated from roots, and NADPH oxidase activity was determined by measuring superoxide anion (O-2(-)) production. NADPH oxidase-dependent O-2(-) production was 11.6 nmol mg(-1) protein min(-1) in control vesicles, but 19.6 nmol after NaCl treatment (24h), indicating that salt stress resulted in the activation of the PM NADPH oxidase. Furthermore, the NaCl-induced increase in total Ca was partially abolished by the addition of 150U/mL catalase (CAT), a H2O2 scavenger, and also by 10 mu M diphenylane iodonium (DPI), a NADPH oxidase inhibitor. This data suggest that NADPH oxidase-dependent H2O2 production might be involved in the modulation of the Ca content in wheat roots. In conclusion, our results show that salinity stress increases the total Ca content of wheat roots, which is partly due to PM NADPH oxidase-dependent H2O2 generation. (C) 2006 Elsevier GmbH. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | CYTOSOLIC-FREE CALCIUM ; PLASMA-MEMBRANE CA2+ ; REACTIVE OXYGEN ; ARABIDOPSIS-THALIANA ; NITRIC-OXIDE ; GUARD-CELLS ; OXIDATIVE STRESS ; SALT-TOLERANCE ; PLANTS ; ACCUMULATION |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000250983300004 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555931 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.NW Normal Univ, Sch Life Sci, Lanzhou 730070, Peoples R China 3.Lanzhou Univ, Sch Life Sci, State Key Lab Arid Agrecol, Lanzhou 730070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yingli,Xua, Shijian,An, Lizhe,et al. NADPH oxidase-dependent hydrogen peroxide production, induced by salinity stress, may be involved in the regulation of total calcium in roots of wheat[J]. JOURNAL OF PLANT PHYSIOLOGY,2007,164(11):1429-1435. |
| APA | Yang, Yingli,Xua, Shijian,An, Lizhe,&Chen, Nianlai.(2007).NADPH oxidase-dependent hydrogen peroxide production, induced by salinity stress, may be involved in the regulation of total calcium in roots of wheat.JOURNAL OF PLANT PHYSIOLOGY,164(11),1429-1435. |
| MLA | Yang, Yingli,et al."NADPH oxidase-dependent hydrogen peroxide production, induced by salinity stress, may be involved in the regulation of total calcium in roots of wheat".JOURNAL OF PLANT PHYSIOLOGY 164.11(2007):1429-1435. |
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来源：寒区旱区环境与工程研究所
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