Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana
文献类型：期刊论文
| 作者 | Wu, J. M.; Zhao, Z. G.; Xing, H.; Guo, H. P.; Li, W. X.; An, L. Z.; Xu, S. J.; Chen, T. |
| 刊名 | BIOLOGIA PLANTARUM
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 51期号:2页码:229-234 |
| 关键词 | alpine plant kinase domain p-nitrophenyl phosphate phosphatase domain |
| ISSN号 | 0006-3134 |
| DOI | 10.1007/s10535-007-0046-4 |
| 通讯作者 | An, L. Z.(lizhean@lzu.edu.cn) |
| 英文摘要 | The influence of freezing treatment on plasma membrane (PM) H+-ATPase was investigated using plasma membrane vesicles isolated from calluses from Chorispora bungeana Fisch. & C.A. Mey. by the discontinuous sucrose gradient centrifugation. Freezing treatment (-4 degrees C) for 5 d resulted in significant increases in the ATPase activity and the activity of p-nitrophenyl phosphate (PNPP) hydrolysis, decreases in the K-m for ATP hydrolysis and PNPP hydrolysis, and the shift of optimal pH from 6.5 to 7.0. Also, the activity PNPP hydrolysis was less sensitive to vanadate after freezing treatment compared to control, while the inhibition of ATP hydrolysis by hydroxylamine was more sensitive. In addition, freezing treatment also decreased the activation effects of trypsin on PNPP hydrolysis, but increased the activation effects of lysophosphatidylcholine on ATP hydrolysis. Taken together, these results suggested that PM H+-ATPase might play an important role during adaptation to freezing and enhancing the frost hardness in C. bungeana. |
| 收录类别 | SCI |
| WOS关键词 | NITROPHENYL PHOSPHATE HYDROLYSIS ; SOYBEAN HYPOCOTYLS ; ROOTS ; CELLS ; LYSOPHOSPHATIDYLCHOLINE ; INVOLVEMENT ; ADAPTATION ; DEFICIENCY ; MODULATION ; ECOTYPES |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000245105100005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555966 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, L. Z. |
| 作者单位 | 1.Lanzhou Univ, Key Lab Arid & Pasture Agroecol, Minist Educ, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Gansu Agr Univ, Coll Agron, Lanzhou 730070, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, J. M.,Zhao, Z. G.,Xing, H.,et al. Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana[J]. BIOLOGIA PLANTARUM,2007,51(2):229-234. |
| APA | Wu, J. M..,Zhao, Z. G..,Xing, H..,Guo, H. P..,Li, W. X..,...&Chen, T..(2007).Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana.BIOLOGIA PLANTARUM,51(2),229-234. |
| MLA | Wu, J. M.,et al."Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana".BIOLOGIA PLANTARUM 51.2(2007):229-234. |
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来源：寒区旱区环境与工程研究所
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