CbLEA, a novel LEA gene from Chorispora bungeana, confers cold tolerance in transgenic tobacco
文献类型：期刊论文
| 作者 | Zhang, Hua; Zhou, Rui Xia; Zhang, Li Jing; Wang, Ruo Yu; An, Li Zhe |
| 刊名 | JOURNAL OF PLANT BIOLOGY
 |
| 出版日期 | 2007-06-30 |
| 卷号 | 50期号:3页码:336-343 |
| 关键词 | chilling stress Chorispora bungeana cold tolerance freezing stress LEA gene transgenic tobacco |
| ISSN号 | 1226-9239 |
| 通讯作者 | An, Li Zhe(lizhean@lzu.edu.cn) |
| 英文摘要 | A novel late embryogenesis abundant (LEA) gene (AY804193), named CbLE4, has now been isolated from Chorispora bungeana. This rare alpine subnival plant can survive sudden snowstorms and low temperatures. The full-length CbLEA is 842 bp, with an open reading frame encoding 169 amino acids. The putative molecular weight of CbLEA protein is 17.9 kDa, with an estimated pi of 6.45. To investigate the functioning of this CbLEA protein in cold-stress tolerance, CbLE4 was introduced into tobacco under the control of the CaMV35S promoter. Second-generation (RI) transgenic tobacco plants exhibited significantly increased tolerance to cold. These transgenics maintained lower malondialdehyde (MDA) contents and electrolyte leakage (EL) but their relative water content (RWC) was significantly higher compared with non-transgenic plants under chilling stress. Further experimental results showed that non-transgenic plants had severe freezing damage after exposure to -2 degrees C for 1 h, whereas the transgenics suffered only slight injury under the same conditions. Moreover, survival was longer in the latter genotype at that temperature. The extent of increased cold tolerance was positive correlated with the level of CbLEA protein accumulation, and was also reflected by the delayed development of damage symptoms. This indicates that CbLE4 is an excellent stress tolerance gene, and holds considerable potential as a new molecular tool for engineering improved plant genetics. |
| 收录类别 | SCI |
| WOS关键词 | FREEZING TOLERANCE ; DROUGHT TOLERANCE ; WATER-DEFICIT ; SALT STRESS ; PROTEIN ; EXPRESSION ; CDNA ; EMBRYOGENESIS ; RICE ; ACID |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000248446500016 |
| 出版者 | BOTANICAL SOC KOREA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555976 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Li Zhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Agroecol, Minist Educ, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hua,Zhou, Rui Xia,Zhang, Li Jing,et al. CbLEA, a novel LEA gene from Chorispora bungeana, confers cold tolerance in transgenic tobacco[J]. JOURNAL OF PLANT BIOLOGY,2007,50(3):336-343. |
| APA | Zhang, Hua,Zhou, Rui Xia,Zhang, Li Jing,Wang, Ruo Yu,&An, Li Zhe.(2007).CbLEA, a novel LEA gene from Chorispora bungeana, confers cold tolerance in transgenic tobacco.JOURNAL OF PLANT BIOLOGY,50(3),336-343. |
| MLA | Zhang, Hua,et al."CbLEA, a novel LEA gene from Chorispora bungeana, confers cold tolerance in transgenic tobacco".JOURNAL OF PLANT BIOLOGY 50.3(2007):336-343. |
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来源：寒区旱区环境与工程研究所
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