Comparison of two gel filtration chromatographic methods for the purification of lily symptomless virus
文献类型：期刊论文
| 作者 | Wang, Ruoyu; Wang, Jianhui; Li, Juan; Wang, Yun; Xie, Zhongkui; An, Lizhe |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 139期号:2页码:125-131 |
| 关键词 | lily symptomless virus purification gel filtration chromatography transmission electron microscope mass spectrometric identification |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2006.09.008 |
| 通讯作者 | An, Lizhe(lizhean@lzu.edu.cn) |
| 英文摘要 | Lily symptomless virus (LSV) occurs frequently in many Lilium species worldwide and often causes developmental abnormalities such as a smaller flower and lower bulb yield. In this study, two moderate and efficient get filtration chromatography (GFC) methods was compared, these two techniques were, respectively, based on Superdex-200 HR and Sephacryl S-1000 SF. The products purified by the two methods were then characterized by measurements with UV-spectrophotometer, reverse transcriptase (RT)-PCR, transmission electron microscope (TEM), polycrylamide gel electrophoresis (PAGE), Western blotting and matrix assisted laser desorption-ionisation/time-of-flight mass spectrometry (MALDI-TOF-MS). The final yield of purified LSV by the Superdex-200 HR GFC was 9.4 mg from 50 g of fresh infected tissues of Lanzhou lily. However, from the same amount tissues, only 5.6 mg of LSV were obtained by using Sephacryl S-1000 SF GFC. The Superdex-200 HR method was thus shown to be more suitable for the purification of LSV than the Sephacryl S-1000 SF GFC. The Superdex-200 HR method does not require costly equipment for density centrifugation and ultracentrifugation. Furthermore, it can provide an economical and efficient way to obtain purified products for the preparation of antibodies for serological diagnosis or LSV infection and related investigations. (c) 2006 Published by Elsevier B.V. |
| 收录类别 | SCI |
| WOS关键词 | MOTTLE-VIRUS ; LILIUM ; PARTICLES ; CARLAVIRUS ; CULTURE ; DISEASE ; PLANTS ; CHINA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244090300001 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2555983 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Sch Life Sci, Minist Educ, Key Lab Arid & Grassland Agroecol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ruoyu,Wang, Jianhui,Li, Juan,et al. Comparison of two gel filtration chromatographic methods for the purification of lily symptomless virus[J]. JOURNAL OF VIROLOGICAL METHODS,2007,139(2):125-131. |
| APA | Wang, Ruoyu,Wang, Jianhui,Li, Juan,Wang, Yun,Xie, Zhongkui,&An, Lizhe.(2007).Comparison of two gel filtration chromatographic methods for the purification of lily symptomless virus.JOURNAL OF VIROLOGICAL METHODS,139(2),125-131. |
| MLA | Wang, Ruoyu,et al."Comparison of two gel filtration chromatographic methods for the purification of lily symptomless virus".JOURNAL OF VIROLOGICAL METHODS 139.2(2007):125-131. |
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来源：寒区旱区环境与工程研究所
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