Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus
文献类型：期刊论文
| 作者 | Wu, Jianmin1; Zhao, Zhiguang1; An, Lizhe1,2; Liu, Yanhong1,3; Xu, Shijian1; Gao, Dahai1; Zhang, Youfu2 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 57期号:1页码:9-17 |
| 关键词 | chilling tolerance Chorispora bungeana fatty acid glutathione plasma membrane |
| ISSN号 | 0011-2240 |
| DOI | 10.1016/j.cryobiol.2008.04.001 |
| 通讯作者 | Wu, Jianmin(wujm03@lzu.edu.cn) |
| 英文摘要 | The possible roles of reduced glutathione(GSH) in chilling tolerance were studied in callus generated from a representative alpine plant, Chorispora bungeana Fisch. & C.A. Mey (C. bungeana). The callus grew well under low-temperature and chilling treatment led only to slight injury, as indicated by a low level of ion leakage (IL). Malondialdehyde measurements also were not elevated, however GSH was. Exogencrusly application of L-buthionine-(S R)-sulfoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase (gamma-ECS), arrested the GSH accumulation induced by chilling and resulted in a significant decrease in cell growth and an increase in IL and malondialdehyde. These results implied that C. bungeana is a plant with a strong low-temperature tolerance mechanism, and the tolerance of C, bungeana may be associated with GSH accumulation. Under chilling treatment, the proportion of unsaturated fatty acid in the plasma membrane (PM) increased significantly in callus of C. bungeana mainly due to increases in C18:2 and C18:3, the membrane fluidity (indicated by DPH fluorescent polarization) however was maintained. High PM H+-ATPase activities were also induced by chilling. Exogenously application of BSO blocked the effects of chilling treatments on the changes of fatty acids and PM H+-ATPase activities, reducing the PM membrane fluidity. On the other hand, simultaneous application of GSH and BSO to callus under chilling treatments reversed the effects of BSO on the changes of fatty acids, PM fluidity and PM H+-ATPase activities. These results Suggested that GSH induced by low-temperature treatments may confer chilling tolerance to C. bungeana, probably by increasing unsaturated fatty acid compositions and maintaining PM fluidity and high enzymatic activity. (C) 2008 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | MEMBRANE H+-ATPASE ; FATTY-ACID-COMPOSITION ; SUSPENSION-CULTURED CELLS ; SENSITIVE MAIZE GENOTYPE ; OXIDATIVE STRESS ; ARABIDOPSIS-THALIANA ; LIPOXYGENASE ACTIVITY ; LOW-TEMPERATURE ; ENVIRONMENTAL SIGNALS ; LIPID-PEROXIDATION |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
| WOS类目 | Biology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258849800002 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556025 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Wu, Jianmin |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Arid & Grassland Agroecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jianmin,Zhao, Zhiguang,An, Lizhe,et al. Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus[J]. CRYOBIOLOGY,2008,57(1):9-17. |
| APA | Wu, Jianmin.,Zhao, Zhiguang.,An, Lizhe.,Liu, Yanhong.,Xu, Shijian.,...&Zhang, Youfu.(2008).Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus.CRYOBIOLOGY,57(1),9-17. |
| MLA | Wu, Jianmin,et al."Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus".CRYOBIOLOGY 57.1(2008):9-17. |
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来源：寒区旱区环境与工程研究所
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