A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA
文献类型：期刊论文
| 作者 | Li, TongXiang1,2; Xie, ZhongKui2; Wang, YaJun2; Li, Bo1; Zhang, YuBo2; An, LiZhe1,2 |
| 刊名 | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
 |
| 出版日期 | 2008-09-01 |
| 卷号 | 62期号:1页码:26-33 |
| 关键词 | detection RNA virus DNA ligation DNA microarray |
| ISSN号 | 0732-8893 |
| DOI | 10.1016/j.diagmicrobio.2008.04.010 |
| 通讯作者 | An, LiZhe(lizhean@izu.edu.cn) |
| 英文摘要 | We describe a novel method that combines RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA. These virus species-specific detection probes (DPs) were designed to match sequences of the "target" virus and then chemically synthesized into 2 parts. If the target virus exists, 2 parts of the DP can be ligated by T4 DNA ligase. The ligated DP was hybridized to its corresponding capture probe (CP) on a DNA array. Then, an on-chip DNA polymerization (including Cy3-dUTP) was performed using the ligated DP as a template and the CP as a primer, which resulted in a reporting fluorescent signal. If the target virus does not exist in a sample, no fluorescence signal is produced. Four common tobacco viruses, tobacco mosaic virus, cucumber mosaic virus, potato virus Y, and potato virus X in single and mixed infections were successfully detected by this method. The sensitivity of the detection limit of this assay is 10 times higher than that of ELISA. The minimum dilution detection limit of this assay was 10(-4) (infected sap/healthy sap). The method has the potential to detect any viral RNA from animal, germs, or fungi where the sequence is known. (C) 2008 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | YELLOW MOSAIC-VIRUS ; LINKED-IMMUNOSORBENT-ASSAY ; PLANT-VIRUSES ; MICROARRAYS ; HYBRIDIZATION ; PROTEINS ; ELISA ; BAYMV ; PCR |
| WOS研究方向 | Infectious Diseases ; Microbiology |
| WOS类目 | Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000258994000006 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556101 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, LiZhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 73000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, TongXiang,Xie, ZhongKui,Wang, YaJun,et al. A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,2008,62(1):26-33. |
| APA | Li, TongXiang,Xie, ZhongKui,Wang, YaJun,Li, Bo,Zhang, YuBo,&An, LiZhe.(2008).A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA.DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,62(1),26-33. |
| MLA | Li, TongXiang,et al."A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA".DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 62.1(2008):26-33. |
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来源：寒区旱区环境与工程研究所
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