Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)
文献类型：期刊论文
| 作者 | Shi, Yulan1; An, Lizhe1,2; Zhang, Manxiao1; Huang, Chenghong1; Zhang, Hua2; Xu, Shijian2 |
| 刊名 | PROTOPLASMA
 |
| 出版日期 | 2008 |
| 卷号 | 232期号:3-4页码:173-181 |
| 关键词 | Chorispora bungeana low temperature plasma membrane plasma membrane H+-ATPase plasma membrane NADH oxidase |
| ISSN号 | 0033-183X |
| DOI | 10.1007/s00709-008-0291-1 |
| 通讯作者 | An, Lizhe(lizhean@lzu.edu.cn) |
| 英文摘要 | As the outermost boundary of the cell, the plasma membrane plays an important role in determining the stress resistance of organisms. To test this concept in a cryophyte, we analyzed alterations of several components in plasma membranes isolated from suspension-cultured cells of Chorispora bungeana Fisch. & C.A. Mey in response to treatment at 0 and -4 degrees C for 192 h. When compared with the controls growing at 25 degrees C, both the membrane permeability and fluidity showed recovery after the initial impairment. Linolenic acid and membrane lipid unsaturation increased by about 0.8-fold following cold treatments, although the kinetics of the increase varied with the temperatures examined. During the treatments, the plasma membrane H+-ATPase (EC 3.6.1.3) activity increased by 78.06% at 0 degrees C and 100.47% at -4 degrees C. However, the plasma membrane NADH oxidase (EC 1.6.99.3) activity only decreased when exposed to a lower temperature (-4 degrees C), and remained at 63.93% after being treated for 192 h. After the treatments, the physical properties of the plasma membranes of suspension-cultured cells, especially the -4 degrees C treated cells, were similar to those in the wild plants. These findings indicate that the specific mechanism of cold resistance of C. bungeana is tightly linked with the rapid and flexible regulation of membrane lipids and membrane-associated enzymes, which ensure the structural and functional integrity of the plasma membrane that is essential for withstanding low temperature. |
| 收录类别 | SCI |
| WOS关键词 | L-CV PUMA ; COLD-ACCLIMATION ; H+-ATPASE ; FREEZING TOLERANCE ; LIPID-COMPOSITION ; FATTY-ACIDS ; SEEDLINGS ; REDOX ; INJURY ; ROOTS |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| WOS类目 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000256803900006 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556123 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yulan,An, Lizhe,Zhang, Manxiao,et al. Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)[J]. PROTOPLASMA,2008,232(3-4):173-181. |
| APA | Shi, Yulan,An, Lizhe,Zhang, Manxiao,Huang, Chenghong,Zhang, Hua,&Xu, Shijian.(2008).Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana).PROTOPLASMA,232(3-4),173-181. |
| MLA | Shi, Yulan,et al."Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana)".PROTOPLASMA 232.3-4(2008):173-181. |
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来源：寒区旱区环境与工程研究所
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