A new potyvirus first isolated and identified from Angelica sinensis
文献类型：期刊论文
| 作者 | Zhang, Yu1; Wang, Ruoyu2; Wang, Jianhui2; Chang, Jiangfeng1; Zhang, Xinfang1; Chen, Tuo2; An, Lizhe1,2; Xu, Shijian1,2 |
| 刊名 | VIRUS GENES
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 39期号:1页码:120-125 |
| 关键词 | Angelica sinensis Potyvirus Peptide mass fingerprinting (PMF) RT-PCR JHMV |
| ISSN号 | 0920-8569 |
| DOI | 10.1007/s11262-009-0361-2 |
| 通讯作者 | Xu, Shijian(xushijian@lzu.edu.cn) |
| 英文摘要 | A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was assigned to the genus Potyvirus, family Potyviridae. Its coat protein (CP) shows high similarity with six other potyviruses by analysis of peptide mass fingerprinting (PMF). The 919 bp nucleotides of 3' terminal covering partial CP gene and 3'-untranslated region was amplified by RT-PCR using degenerate primers which were designed according to the result of PMF. In sequence comparisons and phylogenetic analysis, the new isolate was found to be closely related to Japanese hornwort mosaic virus (JHMV), Konjak mosaic virus (KoMV), and Zantedeschia mosaic virus (ZaMV). The most closely related virus is JHMV03 (AB251346), with 96.59% aa and 87.60% nt identity to the isolate. All results suggest the presence of a new member of potyvirus, tentatively named Dang Gui strain of Japanese hornwort mosaic virus (JHMV-DG*). In our research the antiserum against the CP of JHMV-DG had also been prepared. To our knowledge, it is the first time that a potyvirus has been isolated and identified in Angelica sinensis. |
| 收录类别 | SCI |
| WOS关键词 | MOSAIC-VIRUS ; PROTEINS ; FAMILY ; CHINA ; GENUS ; RNA |
| WOS研究方向 | Genetics & Heredity ; Virology |
| WOS类目 | Genetics & Heredity ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000267299900015 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556149 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Xu, Shijian |
| 作者单位 | 1.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Minist Educ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Wang, Ruoyu,Wang, Jianhui,et al. A new potyvirus first isolated and identified from Angelica sinensis[J]. VIRUS GENES,2009,39(1):120-125. |
| APA | Zhang, Yu.,Wang, Ruoyu.,Wang, Jianhui.,Chang, Jiangfeng.,Zhang, Xinfang.,...&Xu, Shijian.(2009).A new potyvirus first isolated and identified from Angelica sinensis.VIRUS GENES,39(1),120-125. |
| MLA | Zhang, Yu,et al."A new potyvirus first isolated and identified from Angelica sinensis".VIRUS GENES 39.1(2009):120-125. |
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来源：寒区旱区环境与工程研究所
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