CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco
文献类型：期刊论文
| 作者 | Si, Jing1; Wang, Jian-hui2; Zhang, Li-jing3; Zhang, Hua1; Liu, Ya-jie1; An, Li-zhe1 |
| 刊名 | JOURNAL OF PLANT BIOLOGY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 52期号:6页码:593-601 |
| 关键词 | Chilling stress Chorispora bungeana Cold acclimation COR15 gene Freezing stress Transgenic tobacco |
| ISSN号 | 1226-9239 |
| DOI | 10.1007/s12374-009-9077-z |
| 通讯作者 | An, Li-zhe(lizhean@lzu.edu.cn) |
| 英文摘要 | Rapid amplification of cDNA ends was performed to isolate cold-regulated CbCOR15 (EF208112) from Chorispora bungeana. This alpine species is distributed in subnival areas. Transcripts were detected in the leaves, but not the roots, of plants acclimated to cold temperatures. Expression was induced at high levels at both 4A degrees C and -4A degrees C. In comparing its deduced protein sequence to that of AtCOR15a (cold-regulated 15a in Arabidopsis thaliana), the N terminus had less homology than the C terminus while still containing a region analogous to the chloroplast-targeted signal peptide of AtCOR15a. We also introduced CbCOR15, with the CaMV 35S promoter, into tobacco. Second-generation (T1) plants had significantly increased tolerance to chilling, as determined by their electrolyte leakage, chlorophyll content, and relative water content. Further freezing-stress experiments showed that the tolerance of transgenic lines was significantly greater than that of the nontransgenics. Although the degree of chilling and freezing tolerance in the transgenic plants was not directly correlated with the accumulated levels of CbCOR15, we could conclude that this gene confers cold tolerance. |
| 收录类别 | SCI |
| WOS关键词 | TEMPERATURE-RESPONSIVE GENE ; ARABIDOPSIS-THALIANA COR15A ; FREEZING TOLERANCE ; MOLECULAR-CLONING ; EXPRESSION ; PROTEIN ; ACCLIMATION ; CHLOROPLAST ; WHEAT ; DROUGHT |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000272375900013 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556242 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Li-zhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Arid & Grassland Agroecol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Si, Jing,Wang, Jian-hui,Zhang, Li-jing,et al. CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco[J]. JOURNAL OF PLANT BIOLOGY,2009,52(6):593-601. |
| APA | Si, Jing,Wang, Jian-hui,Zhang, Li-jing,Zhang, Hua,Liu, Ya-jie,&An, Li-zhe.(2009).CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco.JOURNAL OF PLANT BIOLOGY,52(6),593-601. |
| MLA | Si, Jing,et al."CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco".JOURNAL OF PLANT BIOLOGY 52.6(2009):593-601. |
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来源：寒区旱区环境与工程研究所
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