Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr.
文献类型：期刊论文
| 作者 | Yang, Yingli1,3; Shi, Ruxia1; Wei, Xueling1; Fan, Qing1; An, Lizhe2,3 |
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 102期号:3页码:387-395 |
| 关键词 | Antioxidant enzyme Calli NADPH oxidase Nitraria tangutorum Bobr. Salinity |
| ISSN号 | 0167-6857 |
| DOI | 10.1007/s11240-010-9745-1 |
| 通讯作者 | Yang, Yingli(xbsfyangyingli@163.com) |
| 英文摘要 | Nitraria tangutorum Bobr., a typical desert halophyte, plays an important ecological role because of its superior tolerance to severe drought and high salinity. Very little is known about the physiological adaptative mechanism of this species to environmental stresses. The aim of this study was to investigate the changes of antioxidant enzyme activities and the regulatory mechanism of ascorbate peroxidase (APX) activity in the calli from Nitraria tangutorum Bobr. after treatment with different NaCl concentrations. The activities of superoxide dismutase (SOD) and catalase (CAT) significantly increased in the calli treated with NaCl, while the peroxidase activity decreased. APX activity was also elevated significantly in response to NaCl, but the increase was partly abolished by H(2)O(2) scavenger dimethylthiourea (DMTU). Furthermore, the excitatory effect of salinity on APX could be alleviated by the addition of exogenous CAT and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor diphenylene iodonium, indicating that the modulation of the APX activity in Nitraria tangutorum Bobr. calli might be associated with NADPH oxidase-dependent H(2)O(2) generation. Measurement and analysis using fluorescent dye 2',7'-dichlorodihydrofluorescein diacetate showed the increase of H(2)O(2) content in salinity-treated calli. The investigation of NADPH-dependent O (2) (-) production in plasma membrane (PM) vesicles isolated from Nitraria tangutorum Bobr. calli revealed that salinity treatment stimulated NADPH oxidase activity. In conclusion, these results suggest that the higher activities of antioxidant enzymes play an important role in the salt tolerance of Nitraria tangutorum Bobr. calli and that the extracellular production of H(2)O(2), depending on the excitation of PM NADPH oxidase, is responsible for enhancing the APX activity in Nitraria tangutorum Bobr. calli under salinity stress. |
| 收录类别 | SCI |
| WOS关键词 | SALT-STRESS ; LIPID-PEROXIDATION ; HYDROGEN-PEROXIDE ; NADPH-OXIDASE ; SUPEROXIDE-DISMUTASE ; OXIDATIVE STRESS ; PLASMA-MEMBRANE ; DEFENSE SYSTEMS ; SUAEDA-SALSA ; TOLERANCE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS类目 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000281761900014 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556384 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Yang, Yingli |
| 作者单位 | 1.NW Normal Univ, Sch Life Sci, Lanzhou 730070, Gansu, Peoples R China 2.Lanzhou Univ, Sch Life Sci, State Key Lab Arid Agroecol, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yingli,Shi, Ruxia,Wei, Xueling,et al. Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr.[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,102(3):387-395. |
| APA | Yang, Yingli,Shi, Ruxia,Wei, Xueling,Fan, Qing,&An, Lizhe.(2010).Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr..PLANT CELL TISSUE AND ORGAN CULTURE,102(3),387-395. |
| MLA | Yang, Yingli,et al."Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr.".PLANT CELL TISSUE AND ORGAN CULTURE 102.3(2010):387-395. |
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来源：寒区旱区环境与工程研究所
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