MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells
文献类型：期刊论文
| 作者 | Wang, Jin; Li, Xinrong; Liu, Yubing; Zhao, Xin; Chen, Cuiyun; Tian, Fang |
| 刊名 | PLANT GROWTH REGULATION
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 63期号:3页码:207-216 |
| 关键词 | Apoptosis Cytochrome c Hydrogen peroxide Mitogen-activated protein kinase Salt stress |
| ISSN号 | 0167-6903 |
| DOI | 10.1007/s10725-010-9517-2 |
| 通讯作者 | Wang, Jin(wangjinlzb@hotmail.com) |
| 英文摘要 | Programmed cell death (PCD) is an active cellular suicide that occurs both in animals and plants throughout development and in response to abiotic or biotic stress. In contrast to plant hypersensitive response-like cell death, little is known about the molecular machinery that regulates the halophyte plant PCD under high salinity stress. Since mitogen-activated protein kinases (MAPKs) are involved in plant response/tolerance to salt stress, and plant MAPK genes belong to the extracellular signal-regulated kinase (ERK) subfamily, we have investigated the role of ERK-like enzymes in high salinity stress-induced cell death in Thellungiella halophila. The data showed that ERK-like enzymes were early (10 min) and transiently activated under 300 mM NaCl stress. Pretreatment with 10 mu M U0126, a special MEK/ERK inhibitor, resulted in a small but statistically significant increase of the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL)-positive nuclei in contrast to salt alone. The effects of U0126 on H(2)O(2) production and cytochrome c (cyt c) release were also investigated. We found that the pretreatment with U0126 accelerated H(2)O(2) production as well as cyt c release, and eventually enhanced cell death. The results suggest that ERK-like enzymes in Thellungiella halophila may act as a positive regulator of salt tolerance, as illustrated by pretreatment with U0126 which enhanced cell death under high salinity stress. |
| 收录类别 | SCI |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; CYTOSOLIC ASCORBATE PEROXIDASE ; CASPASE-LIKE PROTEASES ; TOBACCO BY-2 CELLS ; ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; POTASSIUM-TRANSPORT ; DISEASE RESISTANCE ; SIGNALING CASCADES ; MULTIPLE LEVELS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000288562100001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556468 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Wang, Jin |
| 作者单位 | Chinese Acad Sci, Shapotou Desert Res & Expt Stn, Extreme Stress Resistance & Biotechnol Lab, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jin,Li, Xinrong,Liu, Yubing,et al. MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells[J]. PLANT GROWTH REGULATION,2011,63(3):207-216. |
| APA | Wang, Jin,Li, Xinrong,Liu, Yubing,Zhao, Xin,Chen, Cuiyun,&Tian, Fang.(2011).MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells.PLANT GROWTH REGULATION,63(3),207-216. |
| MLA | Wang, Jin,et al."MEK/ERK inhibitor U0126 enhanced salt stress-induced programmed cell death in Thellungiella halophila suspension-cultured cells".PLANT GROWTH REGULATION 63.3(2011):207-216. |
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来源：寒区旱区环境与工程研究所
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