Brassinosteroids regulate pectin methylesterase activity and AtPME41 expression in Arabidopsis under chilling stress
文献类型：期刊论文
| 作者 | Qu, Tao1; Liu, Rongfang1; Wang, Wei1; An, Lizhe1; Chen, Tuo2; Liu, Guangxiu2; Zhao, Zhiguang1 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 63期号:2页码:111-117 |
| 关键词 | Pectins Pectin methylesterases Brassinosteroid Chilling stress AtPME41 |
| ISSN号 | 0011-2240 |
| DOI | 10.1016/j.cryobiol.2011.07.003 |
| 通讯作者 | An, Lizhe(Lizhean@lzu.edu.cn) |
| 英文摘要 | Pectin methylesterases (PMEs) are important cell wall enzymes that may play important roles in plant chilling/freezing tolerance. We investigated the possible roles of brassinosteroids (BRs) in regulation of PMEs under chilling stress. Chilling stress or 24-epibrassinolide (eBL) treatments induced significant increases in PME activity in wild type (Col-0) seedlings of Arabidopsis. The chilling-stress-induced increases in PME activity were also found in bzr1-D mutant, a BZR1 stabilized mutant with a constitutively active BR signaling pathway, but not in bri1-116, a BR insensitive null allele of the BR receptor BRI1. The results suggest that the regulation of PME activity in Arabidopsis under chilling stress depends on the BR signaling pathway. Furthermore, we showed that the effect of chilling stress on PME activity was impaired in pme41, a knockout mutant of AtPME41. Semi-quantitative RT-PCR results showed that expression of AtPME41 was induced by chilling stress in wild type plants but not in the bri1-116 mutant. The expression of AtPME41 increased in bzr1-D and eBL treated wild type seedlings, but decreased in bri1-116 seedlings. Furthermore, ion leakage induced by low temperature were dramatically increased in both bri1-116 and pme41, while lipid peroxidation was increased in bri1-116 only. The results suggest that BRs may modulate total PME activity in Arabidopsis under chilling stress by regulating AtPME41 expression. Regulation of PME activity may serve as one of the mechanisms that BR participates in chilling tolerance of plants. (C) 2011 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | CELL-WALL ; COLD-ACCLIMATION ; GENE-EXPRESSION ; PLANT-TISSUES ; WINTER-WHEAT ; SIGNAL-TRANSDUCTION ; FREEZING TOLERANCE ; CONFERS TOLERANCE ; ABIOTIC STRESSES ; ABSCISIC-ACID |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
| WOS类目 | Biology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295542600008 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556557 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | An, Lizhe |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Agroecol MOE, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 73000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Tao,Liu, Rongfang,Wang, Wei,et al. Brassinosteroids regulate pectin methylesterase activity and AtPME41 expression in Arabidopsis under chilling stress[J]. CRYOBIOLOGY,2011,63(2):111-117. |
| APA | Qu, Tao.,Liu, Rongfang.,Wang, Wei.,An, Lizhe.,Chen, Tuo.,...&Zhao, Zhiguang.(2011).Brassinosteroids regulate pectin methylesterase activity and AtPME41 expression in Arabidopsis under chilling stress.CRYOBIOLOGY,63(2),111-117. |
| MLA | Qu, Tao,et al."Brassinosteroids regulate pectin methylesterase activity and AtPME41 expression in Arabidopsis under chilling stress".CRYOBIOLOGY 63.2(2011):111-117. |
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来源：寒区旱区环境与工程研究所
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