A combined approach using ISSR and ITS analysis for the characterization of Artemisia halodendron from Horqin sandy land, northern China
文献类型：期刊论文
| 作者 | Huang, Wen-da1,3; Zhao, Xue-yong1,2; Zhao, Xin2; Zhao, Ha-lin1,2; Wang, Shao-kun1,3; Lian, Jie1,3 |
| 刊名 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 39期号:4-6页码:346-351 |
| 关键词 | Artemisia halodendron Habitats gradient Genetic diversity Differentiation ISSR ITS |
| ISSN号 | 0305-1978 |
| DOI | 10.1016/j.bse.2011.04.011 |
| 通讯作者 | Huang, Wen-da(huangwenda2008@yahoo.cn) |
| 英文摘要 | Artemisia halodendron is the most dominant and constructive species among the Horqin sandy land. We evaluated its genetic variation and phylogenetic analysis within and among its populations sampled from six different habitats gradient from the Horqin sandy land in the northeast of China by using inter-simple sequence repeat polymorphism (ISSR) and the nuclear ribosomal internal transcribed spacer (ITS) molecular markers. The results showed that eight ISSR primers generated 84 bands, of which 48 (57.14%) were polymorphic. The highest genetic diversity was observed in the inter-dune lowland population, whereas the lowest diversity was found in the mobile dune population. The AMOVA analysis revealed a relatively high level of genetic variation within its populations. The alignment of 124 ITS sequences showed 401 variable sites and the GC content ranged from 54.02% to 57.32%. The ITS tree revealed the existence of three major clades, and that the semi-mobile dune population was more closely related to the inter-dune lowland population. (C) 2011 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| WOS关键词 | SOIL PROPERTIES ; RESTORATION ; HETEROGENEITY ; POPULATIONS ; TOPOGRAPHY ; VEGETATION ; GRASSLAND ; HABITATS ; PATTERNS ; NITROGEN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000297601400014 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556607 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Huang, Wen-da |
| 作者单位 | 1.Chinese Acad Sci, Naiman Desertificat Res Stn, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Extreme Stress Resistance & Biotechnol Lab, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wen-da,Zhao, Xue-yong,Zhao, Xin,et al. A combined approach using ISSR and ITS analysis for the characterization of Artemisia halodendron from Horqin sandy land, northern China[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2011,39(4-6):346-351. |
| APA | Huang, Wen-da,Zhao, Xue-yong,Zhao, Xin,Zhao, Ha-lin,Wang, Shao-kun,&Lian, Jie.(2011).A combined approach using ISSR and ITS analysis for the characterization of Artemisia halodendron from Horqin sandy land, northern China.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,39(4-6),346-351. |
| MLA | Huang, Wen-da,et al."A combined approach using ISSR and ITS analysis for the characterization of Artemisia halodendron from Horqin sandy land, northern China".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 39.4-6(2011):346-351. |
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来源：寒区旱区环境与工程研究所
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