Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Zhao, Pengshan1,2; Liu, Fei2; Ma, Miao2; Gong, Jiao2; Wang, Qiujun2; Jia, Pengfei2; Zheng, Guochang2; Liu, Heng2 |
| 刊名 | MOLECULAR BIOLOGY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 45期号:5页码:785-796 |
| 关键词 | ABA AtLEA3-3 osmotic stress salt stress seedling establishment transgenic plant |
| ISSN号 | 0026-8933 |
| DOI | 10.1134/S0026893311050165 |
| 通讯作者 | Zhao, Pengshan() |
| 英文摘要 | Previous studies have shown that the late embryogenesis abundant (LEA) group 3 proteins significantly respond to changes in environmental conditions. However, reports that demonstrate their biological role, especially in Arabidopsis, are notably limited. This study examines the functional roles of the Arabidopsis LEA group 3 proteins AtLEA3-3 and AtLEA3-4 in abiotic stress and ABA treatments. Expression of AtLEA3-3 and AtLEA3-4 is upregulated by ABA, high salinity, and osmotic stress. Results on the ectopic expression of AtLEA3-3 and AtLEA3-4 in E. coli suggest that both proteins play important roles in resistance to cold stress. Overexpression of AtLEA3-3 in Arabidopsis (AtLEA3-3-OE) confers salt and osmotic stress tolerance that is characterized during germination and early seedling establishment. However, AtLEA3-3-OE lines show sensitivity to ABA treatment during early seedling development. These results suggest that accumulation of AtLEA3-3 mRNA and/or proteins may help heterologous ABA reinitiate second dormancy during seedling establishment. Analysis of yellow fluorescent fusion proteins localization shows that AtLEA3-3 and AtLEA3-4 are mainly distributed in the ER and that AtLEA3-3 also localizes in the nucleus, and in response to salt, mannitol, cold, or BFA treatments, the localization of AtLEA3-3 and AtLEA3-4 is altered and becomes more condensed. Protein translocalization may be a positive and effective strategy for responding to abiotic stresses. Taken together, these results suggest that AtLEA3-3 has an important function during seed germination and seedling development of Arabidopsis under abiotic stress conditions. |
| 收录类别 | SCI |
| WOS关键词 | EMBRYOGENESIS ABUNDANT PROTEIN ; RESPONSIVE GENE-EXPRESSION ; TRANSCRIPTION FACTOR ; LEA PROTEINS ; WATER-DEFICIT ; FUNCTIONAL-ANALYSIS ; SALT TOLERANCE ; IN-VITRO ; DESICCATION ; GERMINATION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295681900010 |
| 出版者 | MAIK NAUKA/INTERPERIODICA/SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556616 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhao, Pengshan |
| 作者单位 | 1.Chinese Acad Sci, Lab Plant Stress Ecophysiol & Biotechnol, Shapotou Desert Res & Expt Stn, Cold & Arid Regions Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China 2.Lanzhou Univ, MOE Key Lab Arid & Grassland Ecol, Inst Cell Biol, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Pengshan,Liu, Fei,Ma, Miao,et al. Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana[J]. MOLECULAR BIOLOGY,2011,45(5):785-796. |
| APA | Zhao, Pengshan.,Liu, Fei.,Ma, Miao.,Gong, Jiao.,Wang, Qiujun.,...&Liu, Heng.(2011).Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana.MOLECULAR BIOLOGY,45(5),785-796. |
| MLA | Zhao, Pengshan,et al."Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana".MOLECULAR BIOLOGY 45.5(2011):785-796. |
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来源：寒区旱区环境与工程研究所
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