Iron-mediated inhibition of H+-ATPase in plasma membrane vesicles isolated from wheat roots
文献类型：期刊论文
| 作者 | Yang, YL; Zhang, F; He, WL; Wang, XM; Zhang, LX |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2003-06-01 |
| 卷号 | 60期号:6页码:1249-1257 |
| 关键词 | H-ATPase plasma membrane iron lipid peroxidation P-SH oxidation wheat |
| ISSN号 | 1420-682X |
| DOI | 10.1007/s00018-003-3093-9 |
| 通讯作者 | Zhang, LX() |
| 英文摘要 | The mechanisms of iron-mediated inhibition of the H-ATPase activity of plasma membrane (PM) vesicles isolated from wheat roots were investigated. Both FeSO4 and FeCl3 significantly inhibited PM H+-ATPase activity, and the inhibition could be reversed by the addition of the metal ion chelator EDTA-Na-2 or a specific Fe2+ chelator, indicating that the inhibitory effect was due to specific action of Fe2+ or Fe3+. Measurement of the extent of lipid peroxidation showed that oxidative damage on the PM caused by Fe2+ or Fe3+ seemed to be correlated with the inhibition of PM H+-ATPase activity. However, prevention of lipid peroxidation with butylated hydroxytoluene did not affect iron-mediated inhibition in the PM H+-ATPase, suggesting that the inhibition of the PM H+-ATPase was not a consequence of lipid peroxidation caused by iron. Investigation of the effects of various reactive oxygen species scavengers on the iron-mediated inhibition of H+-ATPase activity indicated that hydroxyl radicals (.OH) and hydrogen peroxide (H2O2) might be involved in the Fe2+-mediated decrease in PM H+-ATPase activity. Moreover, iron caused a decrease in plasma protein thiol (P-SH), and Fell brought a higher degree of oxidation in thiol groups than Fe2+ at the same concentration. Modification of the thiol redox state in the PM suggested that reducing thiol groups were essential to maintain PM H+-ATPase activity. Incubation of the specific thiol modification reagent 5,5-dithio-bis(2-nitrobenzoic acid) with the rightside-out and inside-out PM revealed that thiol oxidation occurred at the apoplast side of the PM. Western blotting analysis revealed a decrease in H+-ATPase content caused by iron. Taken together, these results suggested that thiol oxidation might account for the inhibition of PM H+-ATPase caused by iron, and that .OH and H2O2 were also involved in Fe2+-mediated inhibition. |
| 收录类别 | SCI |
| WOS关键词 | STRESS ; INDUCTION ; PROTEINS ; PLANTS ; DAMAGE ; GROWTH ; WELL ; LEAF ; NACL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000184007200019 |
| 出版者 | BIRKHAUSER VERLAG AG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556842 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Zhang, LX |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Desert & Desertificat, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, State Key Lab Arid Agroecol, Sch Life Sci, Lanzhou 730000, Peoples R China 3.NW Normal Univ, Sch Life Sci, Lanzhou 730070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, YL,Zhang, F,He, WL,et al. Iron-mediated inhibition of H+-ATPase in plasma membrane vesicles isolated from wheat roots[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2003,60(6):1249-1257. |
| APA | Yang, YL,Zhang, F,He, WL,Wang, XM,&Zhang, LX.(2003).Iron-mediated inhibition of H+-ATPase in plasma membrane vesicles isolated from wheat roots.CELLULAR AND MOLECULAR LIFE SCIENCES,60(6),1249-1257. |
| MLA | Yang, YL,et al."Iron-mediated inhibition of H+-ATPase in plasma membrane vesicles isolated from wheat roots".CELLULAR AND MOLECULAR LIFE SCIENCES 60.6(2003):1249-1257. |
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来源：寒区旱区环境与工程研究所
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