Blue native gel electrophoresis analysis of chloroplast pigment protein complexes
文献类型：期刊论文
| 作者 | Li, BB; Guo, JK; Zhou, Y; Zhang, ZZ; Zhang, LX |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2003-08-01 |
| 卷号 | 30期号:4页码:639-643 |
| 关键词 | blue-native polyacrylamide gel electrophoresis thylakoid membrane protein complexes Western blotting |
| ISSN号 | 1000-3282 |
| 英文摘要 | Blue-native polyacrylamide gel electrophoresis is a very powerful procedure that can be used to analyze the components of chloroplast protein complexes. The two photosystems, the ATP synthase, the cytochrome complex b(6)f complex, the light-harvesting complexes and the RubisCO are well resolved. Analysis of chloroplast protein on a second gel dimension allows seperation of more than 50 different proteins which are part of multi-subunit enzymes. Western blotting analysis was also used to confirm the identity of the complex. The method has been used to analyze protein complexes of grana and stromal thylakoid. |
| 收录类别 | SCI |
| WOS关键词 | D1 PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000184947500026 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2556922 |
| 专题 | 寒区旱区环境与工程研究所 |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, BB,Guo, JK,Zhou, Y,et al. Blue native gel electrophoresis analysis of chloroplast pigment protein complexes[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(4):639-643. |
| APA | Li, BB,Guo, JK,Zhou, Y,Zhang, ZZ,&Zhang, LX.(2003).Blue native gel electrophoresis analysis of chloroplast pigment protein complexes.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(4),639-643. |
| MLA | Li, BB,et al."Blue native gel electrophoresis analysis of chloroplast pigment protein complexes".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.4(2003):639-643. |
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来源：寒区旱区环境与工程研究所
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