Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow
文献类型：期刊论文
| 作者 | Liang SL; Fu ZL(傅长亮) ; Wagner D; Guo HG; Zhan DY; Dong C; Long M(龙勉)
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| 刊名 | American Journal of Physiology-Cell Physiology
 |
| 出版日期 | 2008 |
| 页码 | C743-C753 |
| 通讯作者邮箱 | cxd23@psu.edu |
| 关键词 | Heterotypic Cell Aggregation Adhesion Molecule Leukocyte Tumor Cell Reverse Rate Binding Affinity Probabilistic Model Polymorpho-Nuclear Neutrophils Intercellular Adhesion Molecule-1 Intercellular-Adhesion Molecule-1 Ligand-Mediated Adhesion Hydrodynamic Shear L-Selectin Malignant-Melanoma Dynamic Regulation Variant Isoforms Stable Adhesion Latex Spheres P-Selectin |
| ISSN号 | 0363-6143 |
| 通讯作者 | Dong, C (reprint author), Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA. |
| 中文摘要 | Cell adhesion, mediated by specific receptor-ligand interactions, plays an important role in biological processes such as tumor metastasis and inflammatory cascade. For example, interactions between beta(2)-integrin ( lymphocyte function-associated antigen-1 and/or Mac-1) on polymorphonuclear neutrophils (PMNs) and ICAM-1 on melanoma cells initiate the bindings of melanoma cells to PMNs within the tumor microenvironment in blood flow, which in turn activate PMN-melanoma cell aggregation in a near-wall region of the vascular endothelium, therefore enhancing subsequent extravasation of melanoma cells in the microcirculations. Kinetics of integrin-ligand bindings in a shear flow is the determinant of such a process, which has not been well understood. In the present study, interactions of PMNs with WM9 melanoma cells were investigated to quantify the kinetics of beta(2)-integrin and ICAM-1 bindings using a cone-plate viscometer that generates a linear shear flow combined with a two-color flow cytometry technique. Aggregation fractions exhibited a transition phase where it first increased before 60 s and then decreased with shear durations. Melanoma-PMN aggregation was also found to be inversely correlated with the shear rate. A previously developed probabilistic model was modified to predict the time dependence of aggregation fractions at different shear rates and medium viscosities. Kinetic parameters of beta(2)-integrin and ICAM-1 bindings were obtained by individual or global fittings, which were comparable to respectively published values. These findings provide new quantitative understanding of the biophysical basis of leukocyte-tumor cell interactions mediated by specific receptor-ligand interactions under shear flow conditions. |
| 类目[WOS] | Cell Biology ; Physiology |
| 研究领域[WOS] | Cell Biology ; Physiology |
| 关键词[WOS] | INTERCELLULAR-ADHESION MOLECULE-1 ; LIGAND-MEDIATED ADHESION ; HYDRODYNAMIC SHEAR ; L-SELECTIN ; MALIGNANT-MELANOMA ; DYNAMIC REGULATION ; VARIANT ISOFORMS ; STABLE ADHESION ; LATEX SPHERES ; INTEGRIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253992300009 |
| 公开日期 | 2009-08-03 ; 2009-09-14 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/26114]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Liang SL,Fu ZL,Wagner D,et al. Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow[J]. American Journal of Physiology-Cell Physiology,2008:C743-C753. |
| APA | Liang SL.,傅长亮.,Wagner D.,Guo HG.,Zhan DY.,...&龙勉.(2008).Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow.American Journal of Physiology-Cell Physiology,C743-C753. |
| MLA | Liang SL,et al."Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow".American Journal of Physiology-Cell Physiology (2008):C743-C753. |
入库方式： OAI收割
来源：力学研究所
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