Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert
文献类型：期刊论文
| 作者 | Wang, Jin1,2; Bao, Jing-Ting1,3,4; Li, Xin-Rong1,2; Liu, Yu-Bing1,2 |
| 刊名 | MICROBIAL ECOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 71期号:1页码:150-163 |
| ISSN号 | 0095-3628 |
| 关键词 | Biological soil crust Chronosequence Diazotrophic community Pyrosequencing Revegetation |
| DOI | 10.1007/s00248-015-0657-9 |
| 通讯作者 | Li, Xin-Rong(lxinrong@lzb.ac.cn) |
| 英文摘要 | The colonization and succession of diazotrophs are essential for the development of organic soil layers in desert. We examined the succession of diazotrophs in the well-established revegetated areas representing a chronosequence of 0 year (control), 22 years (restored artificially since 1981), 57 years (restored artificially since 1956), and more than 100 years (restored naturally) to determine the community assembly and active expression of diazotrophs. The pyrosequencing data revealed that Alphaproteobacteria-like diazotrophs predominated in the topsoil of our mobile dune site, while cyanobacterial diazotrophs predominated in the revegetated sites. The cyanobacterial diazotrophs were primarily composed of the heterocystous genera Anabaena, Calothrix, Cylindrospermum, Nodularia, Nostoc, Trichormus, and Mastigocladus. Almost all the nifH sequences belonged to the Cyanobacteria phylum (all the relative abundance values > 99.1 %) at transcript level and all the active cyanobacterial diazotrophs distributed in the families Nostocaceae and Rivulariaceae. The most dominant active cyanobacterial genus was Cylindrospermum in all the samples. The rank abundance and community analyses demonstrated that most of the diazotrophic diversity originated from the "rare" species, and all the DNA-based diazotrophic libraries were richer and more diverse than their RNA-based counterparts in the revegetated sites. Significant differences in the diazotrophic community and their active population composition were observed among the four research sites. Samples from the 1981-revegetating site (predominated by cyanobacterial crusts) showed the highest nitrogenase activity, followed by samples from the naturally revegetating site (predominated by lichen crusts), the 1956-revegetating site (predominated by moss crusts), and the mobile dune site (without crusts). Collectively, our data highlight the importance of nitrogen fixation by the primary successional desert topsoil and suggest that the N-2-fixing cyanobacteria are the key diazotrophs to the nitrogen budget and the development of topsoil in desert, which is critical for the succession of the degraded terrestrial ecosystems. |
| 收录类别 | SCI |
| WOS关键词 | BIOLOGICAL SOIL CRUSTS ; NITROGEN-FIXATION ; COLORADO PLATEAU ; NORTHERN CHINA ; TRICHODESMIUM-THIEBAUTII ; DIAZOTROPHIC COMMUNITY ; BACTERIAL DIVERSITY ; SUCCESSIONAL STAGES ; INORGANIC NITROGEN ; CHIHUAHUAN DESERT |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology |
| WOS类目 | Ecology ; Marine & Freshwater Biology ; Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000367097500015 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2557151 |
| 专题 | 寒区旱区环境与工程研究所 |
| 通讯作者 | Li, Xin-Rong |
| 作者单位 | 1.Chinese Acad Sci, Shapotou Desert Expt & Res Stn, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lab Plant Stress Ecophysiol & Biotechnol, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jin,Bao, Jing-Ting,Li, Xin-Rong,et al. Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert[J]. MICROBIAL ECOLOGY,2016,71(1):150-163. |
| APA | Wang, Jin,Bao, Jing-Ting,Li, Xin-Rong,&Liu, Yu-Bing.(2016).Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert.MICROBIAL ECOLOGY,71(1),150-163. |
| MLA | Wang, Jin,et al."Molecular Ecology of nifH Genes and Transcripts Along a Chronosequence in Revegetated Areas of the Tengger Desert".MICROBIAL ECOLOGY 71.1(2016):150-163. |
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来源：寒区旱区环境与工程研究所
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